KEGG ENZYME: 1.12.1.4

ENZYME: 1.12.1.4

Entry

EC 1.12.1.4                 Enzyme

Name

hydrogenase (NAD+, ferredoxin);
bifurcating [FeFe] hydrogenase

Class

Oxidoreductases;
Acting on hydrogen as donor;
With NAD+ or NADP+ as acceptor

Sysname

hydrogen:NAD+, ferredoxin oxidoreductase

Reaction(IUBMB)

2 H2 + NAD+ + 2 oxidized ferredoxin = 5 H+ + NADH + 2 reduced ferredoxin [RN:R09508]

Reaction(KEGG)

R09508

Substrate

H2 [CPD:C00282];
NAD+ [CPD:C00003];
oxidized ferredoxin [CPD:C00139]

Product

H+ [CPD:C00080];
NADH [CPD:C00004];
reduced ferredoxin [CPD:C00138]

Comment

The enzyme from Thermotoga maritima contains a [FeFe] cluster (H-cluster) and iron-sulfur clusters. It works in the direction evolving hydrogen as a means of eliminating excess reducing equivalents.

History

EC 1.12.1.4 created 2011

Orthology

K17997

iron-hydrogenase subunit alpha

K17998

iron-hydrogenase subunit beta

K17999

iron-hydrogenase subunit gamma

Genes

DMP:

FAK_02870

GEX:	GETHOR_11520

MSIL:

METEAL_03860 METEAL_24040

MSEA:

METESE_03930 METESE_17650 METESE_17710

DTH:	DICTH_0410 DICTH_0411 DICTH_0412

DTU:	Dtur_0558 Dtur_0559 Dtur_0560

ALAM:

RT761_01588(hndA_1)

TVX:	QBE54_05645(nuoE)

ATHO:

M15_09590(nuoE_1)

CFER:

D4Z93_04795(nuoE)

CPRF:

K7H06_11925

CRAS:

KVH43_02035(nuoE)

PROQ:

P6M73_16530

RPAY:

P0092_04410

PFAA:

MM59RIKEN_16600

VFA:	MM35RIKEN_11540

PETR:

QKW49_16090

OKO:	Ruko_23390

ELIM:

B2M23_16035

PROM:

QO263_06545(nuoE)

BCOC:

BLCOC_31380(nuoE_1)

CSCI:

HDCHBGLK_01919(nuoE_2)

SCAG:

ACI3DM_06550

AHB:	bsdtb5_32680

AMIC:

Ami3637_14050(nuoE)

ABUT:

Ami103574_11155(nuoE)

ETM:	CE91St48_17640

EMJ:	V1225_10730(nuoE)

AHAD:

FRZ06_03730 FRZ06_07135(nuoE) FRZ06_08270(nuoE) FRZ06_12770

CSPO:

QNI18_06940(nuoE)

GEK:	kuro4_25180 kuro4_25200

NCD:	ACONDI_01150(hndA_3)

KME:	H0A61_01404(hndA_2) H0A61_01684(hndA_3)

HPRF:

HLPR_21220(nuoE_4)

TIZ:	RBU61_13730

SEDM:

RBQ61_10410

SSPH:

SPSPH_007540(hndA_1) SPSPH_014000(hndA_3)

SSID:

SPSIL_016320(hndA_1)

SACV:

SPACI_019160(hndA_1)

CALK:

HUE98_04290 HUE98_14100

CNAG:

OTJ99_000389(nuoE)

MANR:

MPAN_008060

TMA:	TM1424 TM1425 TM1426

TMM:	Tmari_1430 Tmari_1431 Tmari_1432

TMI:	THEMA_07185 THEMA_07190 THEMA_07195

TMW:	THMA_1454 THMA_1455 THMA_1456

TMQ:	THMB_1454 THMB_1455 THMB_1456

TMX:	THMC_1454 THMC_1455 THMC_1456

TPT:	Tpet_1367 Tpet_1368 Tpet_1369

TNP:	Tnap_1385 Tnap_1386 Tnap_1387

TRQ:	TRQ2_1317 TRQ2_1318 TRQ2_1319

TNA:	CTN_1067 CTN_1068 CTN_1069

THQ:	T2812B_07055 T2812B_07060 T2812B_07065

THZ:	CELL2_07115 CELL2_07120 CELL2_07125

THR:	TRQ7_06790 TRQ7_06795 TRQ7_06800

TLE:	Tlet_0952 Tlet_0953 Tlet_0954

TTA:	Theth_0097 Theth_0098 Theth_0099

PHY:	AJ81_06395 AJ81_06400 AJ81_06405

TME:	Tmel_0490 Tmel_0491 Tmel_0492

TAF:	THA_785 THA_786 THA_787

THP:	BG95_03210 BG95_03215 BG95_03220

THER:

Y592_03220 Y592_03225 Y592_03230

TFE:	JYK00_03185(nuoE) JYK00_03190 JYK00_03195

FNO:	Fnod_0225 Fnod_0226 Fnod_0227

FPE:	Ferpe_0860 Ferpe_0861 Ferpe_0862

FIA:	NA23_04200(nuoE) NA23_04205 NA23_04210

FRR:	IB67_06725 IB67_06730 IB67_06735

FNG:	JM64_02660 JM64_02665 JM64_02670

MESB:

L1S32_11290

» show all

Reference

1 [PMID:10482784]

Authors

Verhagen MF, O'Rourke T, Adams MW

Title

The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization.

Journal

Biochim Biophys Acta 1412:212-29 (1999)
DOI:10.1016/S0005-2728(99)00062-6

Sequence

[tma:TM1426 TM1425 TM1424]

Reference

2 [PMID:19411328]

Authors

Schut GJ, Adams MW

Title

The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production.

Journal

J Bacteriol 191:4451-7 (2009)
DOI:10.1128/JB.01582-08