Entry Name 3-oxocholoyl-CoA 4-desaturase;
Class Oxidoreductases;BRITE hierarchy
Sysname 3-oxocholoyl-CoA Delta4-oxidoreductase
Reaction(IUBMB) (1) 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:
R12109 ];
(2) 7alpha-hydroxy-3-oxochol-24-oyl-CoA + NAD+ = 7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ [RN:
R12110 ]
Reaction(KEGG) Substrate 7alpha,12alpha-dihydroxy-3-oxochol-24-oyl-CoA [CPD:
C21910 ];
NAD+ [CPD:
C00003 ];
7alpha-hydroxy-3-oxochol-24-oyl-CoA [CPD:
C21909 ]
Product 7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:
C21469 ];
NADH [CPD:
C00004 ];
H+ [CPD:
C00080 ];
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA [CPD:
C21471 ]
Comment Contains flavin. The enzyme, characterized from the bacterium Clostridium scindens, participates in the bile acid 7alpha-dehydroxylation pathway. The enzyme catalyses the stereo-specific oxidation of its substrates and has no activity with the 7beta anomers. cf. EC
1.3.1.116 , 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase.
History EC 1.3.1.115 created 2018
Pathway ec00121 Secondary bile acid biosynthesis
Orthology K15870 3-oxocholoyl-CoA 4-desaturase
Genes Taxonomy Reference Authors Kang DJ, Ridlon JM, Moore DR 2nd, Barnes S, Hylemon PB
Title Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-delta4-cholenoic acid oxidoreductases.
Journal Sequence Other DBs ExPASy - ENZYME nomenclature database: 1.3.1.115
LinkDB All DBs
