KEGG ENZYME: 1.3.1.77

ENZYME: 1.3.1.77

Entry

EC 1.3.1.77                 Enzyme

Name

anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming];
ANR (gene name) (ambiguous);
flavan-3-ol:NAD(P)+ oxidoreductase;
anthocyanidin reductase (ambiguous)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase

Reaction(IUBMB)

a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]

Reaction(KEGG)

R07352 > R06541 R06542 R06543;
R07351

Substrate

(2R,3R)-flavan-3-ol [CPD:C22577];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]

Product

anthocyanidin with a 3-hydroxy group [CPD:C21381];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].

History

EC 1.3.1.77 created 2004, modified 2016

Pathway

ec00941

Flavonoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K08695

anthocyanidin reductase

Genes

ATH:	AT1G61720(BAN)

ALY:

9322580

CRB:

17894009

CSAT:

104703765 104714500 104752826

EUS:	EUTSA_v10023567mg

BRP:	103831042 103838541

BNA:	106352233 106374812 106374840 106395425

BOE:	106302532 106323258 106341298

RSZ:	108810876 108828925 130494385

THJ:

104809521

CPAP:

110812199

CIT:

102618954

CIC:	CICLE_v10017667mg

PVY:	116105178 116141969

MINC:

123219441 123219660

TCC:	18597033(ANR)

GRA:

105768219

GHI:	107905961 107938221

GAB:

108452981

HSYR:

120131627 120190784

DZI:

111280787

EGR:	104441057 104441153

GMX:	100805127(ANR1) 100817081(ANR2)

GSJ:	114421336 114422060

PVU:	137809756 137811942

VRA:	106766353 106767189 106767332 106773404

VAR:	108323842 108337959

VUN:	114176923 114179528

VUM:	124822721 124830583 124841586 124841588

CCAJ:

109794672 109817877

APRC:

113858180 113859920

MTR:

25493251

TPRA:

123918149

CAM:

101494604

PSAT:

127100664

VVO:

131602777

LJA:

130715053

ADU:

107480609

AIP:

107626250

AHF:	112728650 112791357

LANG:

109330732

PCIN:

129297719 129304631

QSA:	O6P43_031731

FVE:

101297977

AANS:

126786175

RCN:

112202296

PPER:

18778549

PMUM:

103324591

PAVI:

110768767

PDUL:

117624652

MDM:	103434638 114827797

MSYL:

126586076 126624200

PXB:	103957769 103957850

PCOX:

137724458 137728805

ZJU:

107421422

MNT:

21397956

CSAV:

115708131

RCU:

8267420

MEAN:

126682263

JCU:

105649408

HBR:

110658083

MESC:

110602917

POP:	7490768 7495295

PEU:

105110148

PALZ:

118042818

PNZ:	133699702 133703010

JRE:

108979344

CILL:

122277370

QSU:

111989132

QLO:	115972793 115992511

TWL:	119990111 120005016

VRI:

117924389

SLY:

101263493

SPEN:

107012467

SOT:

102584577

SSTN:

125860515

SDUL:

129894901

LBB:

132617081

NTA:	107770662 107829462

NSY:

104233566

NTO:

104120432

NAU:

109243327

CSIN:

114259095 114297899

RVL:

131313714

BVG:

104883056

SOE:

130464271

CQI:	110687076 110693741

ATRI:

130815880

NNU:	104585744 104593362 104604944

MING:

122062392 122091471

TSS:	122639738 122647935

PSOM:

113318553 113348609

OSA:	4337098 4337100 4337101 4337102

DOSA:

Os04g0630600 Os04g0630800 Os04g0630900 Os04g0631000

OBR:	102705034 102716961 102717241

OGL:	127770157 127770943

BDI:	100834459 100834762 100835070

ATS:	109733882 109736125 109736130 109736133 109738472 109750081 109766837 109778519

TDC:

119273584 119340433 119343101 119343446 119344362 119344363 119344594 119345496 119348096 119348639 119352459 119356613 119356614 119356615 119356617 119356618 119356619 119356622 119356623 119356625 119356627 119357206 119357207 119358703 119361244 119365826 119365829 119365830 119365831 119365832 119365836 119365837 119366539 119366540 119368461

TAES:

123040141 123042234 123042237 123042239 123042851 123046886 123046888 123046889 123046890 123046891 123047541 123047542 123050175 123050279 123054723 123054724 123054727 123054732 123054733 123054738 123186210 123186211 123186212 123186651 123186652 123186653 123190564 123190566 123190569 123190572 123190573 123191123 123191124

TUA:	125527806 125527807 125527876 125527877 125528738 125530497 125533915 125534183 125534191 125534196 125534197 125534569 125539841 125539842 125539844 125539845 125546262

LPER:

127319508 127330289 127335043 127336090 127336091 127336094

LRD:	124655259 124674400 124674401 124674734 124675431 124675465 124675466 124676098 124676099 124676100 124677538

SBI:	110436259 8066840 8066841 8066842 8066844 8066846

ZMA:	100191338 100280098 100282500 103652362 118474966

MFLO:

136487559 136491511 136491512 136491514 136491516 136491517 136491518 136497603 136497604 136497605 136497608 136497609 136497611

SITA:

101757899 101758322 101759132 101784129

SVS:

117863580

PVIR:

120641879 120641880 120641882 120641883 120644933 120675175 120675466 120675567 120676510 120681344 120681438 120682923 120682924

PHAI:

112899176 112899177 112899179

PAUA:

133888134 133888135 133888136 133888137 133888139 133888141 133888142 133888998 133888999 133925051 133925053

PDA:

103698208

EGU:

105035639

MUS:

135618147

ZOF:	121973714 121977884 122011404 122016036

AOF:

109821630

MSIN:

131221638 131221640 131221641 131221642 131221643 131221645 131221647 131221650 131221651 131221652 131236075 131237805

ACAK:

137942596 137942597 137942598 137951454

NCOL:

116263884

ATR:

18427888

CJF:

131056615

» show all

Reference

1 [PMID:12532018]

Authors

Xie DY, Sharma SB, Paiva NL, Ferreira D, Dixon RA

Title

Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis.

Journal

Science 299:396-9 (2003)
DOI:10.1126/science.1078540

Sequence

[ag:AAN77735]

Reference

2 [PMID:14725861]

Authors

Xie DY, Sharma SB, Dixon RA.

Title

Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana.

Journal

Arch Biochem Biophys 422:91-102 (2004)
DOI:10.1016/j.abb.2003.12.011

Sequence

[ath:AT1G61720]

Reference

3 [PMID:23288883]

Authors

Pang Y, Abeysinghe IS, He J, He X, Huhman D, Mewan KM, Sumner LW, Yun J, Dixon RA

Title

Functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering.

Journal

Plant Physiol 161:1103-16 (2013)
DOI:10.1104/pp.112.212050

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.77

IUBMB Enzyme Nomenclature:

1.3.1.77

ExPASy - ENZYME nomenclature database:

1.3.1.77

BRENDA, the Enzyme Database:

1.3.1.77

CAS:	93389-48-1

LinkDB

DBGET integrated database retrieval system