KEGG ENZYME: 1.6.3.4

ENZYME: 1.6.3.4

Entry

EC 1.6.3.4                  Enzyme

Name

NADH oxidase (H2O-forming);
H2O-forming NADH oxidase;
Nox-2

Class

Oxidoreductases;
Acting on NADH or NADPH;
With oxygen as acceptor

Sysname

NADH:oxygen oxidoreductase (H2O-forming)

Reaction(IUBMB)

2 NADH + 2 H+ + O2 = 2 NAD+ + 2 H2O [RN:R10517]

Reaction(KEGG)

R10517

Substrate

NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). The bacterium Streptococcus mutans contains two distinct NADH oxidases, a H2O-forming enzyme and a H2O2-forming enzyme (cf. EC 1.6.3.3, NADH oxidase (H2O2-forming)) [1].

History

EC 1.6.3.4 created 2013

Orthology

K17869

NADH oxidase (H2O-forming)

K22405

NADH oxidase (H2O-forming)

Genes

MMT:	Metme_1624

MDN:	JT25_005995

MDH:	AYM39_16345

MKO:	MKLM6_3358

METL:

U737_04005

MPAD:

KEF85_00880

MELL:

IVG45_12825

MMOT:

QZJ86_04550

MEEP:

JWZ98_03595

MEEC:

PL263_09505

MEUP:

QC632_20015

MBUR:

EQU24_17220

MPSY:

CEK71_14720

MMOB:

F6R98_01550

MOZ:	MoryE10_26400(fprA)

MESL:

KKZ03_21000

TIG:

THII_3128

BLEP:

AL038_16445

THIS:

HZT40_09015

TUN:	J9260_05615

TLO:	J9253_07425

TSB:	HMY34_12970

TSJ:	L3K52_17600

TVI:	Thivi_2130

TMB:	Thimo_3250

TSY:	THSYN_03990

THIP:

N838_15565

CJAP:

GWK36_11795

THIM:

KFB96_17595

TBOG:

LT988_12130

TLR:	Thiosp_01216(fprA)

TFRI:

Thiofri_01529(fprA_1)

EBS:	ECTOBSL9_2540

AQL:	BXU06_13035

SNIV:

SFSGTM_04400(fprA)

DAR:

Daro_1451

DEY:	HYN24_06790

DDEN:

KI615_08700

QAU:	KI612_04970

FLE:	KI610_08875

AHV:	AADV58_06390

AZO:	azo0526(fprA)

AOA:

dqs_0536

AZR:	CJ010_03500

AZQ:	G3580_18960

ACOM:

CEW83_10515

APP:	CAP2UW1_4425

RSP:	RSP_3191(fprA)

RSH:	Rsph17029_3930

RSQ:	Rsph17025_3191

RSK:	RSKD131_3367

CAZT:

LV780_15550

RHC:

RGUI_2055

DAA:	AKL17_1541

RCP:	RCAP_rcc03286(norV)

WSU:

WS0163

SBAL:

HUE88_05590

SMAX:

FJR03_02795

SKU:	Sulku_1316

SBA:	Sulba_0996

SMUL:

SMUL_1215

SHAL:

SHALO_1200

SULS:

Sdiek1_1284

SULJ:

SJPD1_1233

SULT:

FA592_12375

ANT:	Arnit_0077

AELL:

AELL_0048

ASUI:

ASUIS_0050

AVP:	AVENP_0064

ADZ:	ADFLV_0072

ARC:

ABLL_0060

PACO:

AACT_0052

HEBR:

AEBR_0103

AANA:

AANAER_0109

SMAB:

LN246_08390

DAV:	DESACE_01655

GPI:	GPICK_15220

GEM:

GM21_0168

GEB:

GM18_0199

GEF:	FO488_18975

GEV:	GSVR_03870(norV)

GUR:

Gura_0371

GEO:	Geob_1311(roo)

GLO:

Glov_0399

TAMM:

GEAMG1_0121(fprA)

GBM:	Gbem_0186(roo)

GBN:	GEOBRER4_01720(norV)

GER:	KP004_20755

GSUB:

KP001_15575

GNT:	KP003_20680

GPL:	M1B72_21175

GSB:	GSUB_03650

GHC:	L9S41_05505

DEU:

DBW_1080

DVE:	DESUT3_31850(fprA)

DEP:	AOP6_0617(fprA)

DES:	DSOUD_0965(roo)

KST:	KSMBR1_2772(fprA)

BROC:

IPI25_12290

BPIT:

BPIT_27170

JET:	L3J17_10755

APS:

CFPG_262

SHYR:

LA303_07220

CTE:

CT2285

CPC:

Cpar_0005

CLZ:	BIU88_00020

CCH:

Cag_2008

CPH:	Cpha266_0026

CPB:	Cphamn1_0006

CLI:

Clim_0005

PVI:

Cvib_0005

PLT:

Plut_0004

PPH:

Ppha_0005

PAA:

Paes_0005

PROC:

Ptc2401_00005(fprA1)

PRS:	B9H02_00025

PROS:

CHL67_00030

PROW:

OO006_00035

PROI:

OO005_00035

DIN:	Selin_0989

DDF:	DEFDS_0572

DAP:	Dacet_1865

GTL:	EP073_09040

CNI:	Calni_0057

FSI:	Flexsi_1655

LCA:

LSEI_0285

LCZ:	LCAZH_0305

LCS:

LCBD_0278

LCE:

LC2W_0269

LCW:	BN194_02850(nox_2)

LCL:	LOCK919_0299

LPQ:	AF91_00725

LPI:	LBPG_02448

LPAP:

LBPC_0268

LCB:	LCABL_02800(nox)

LCX:	LCA12A_2517

LRH:	LGG_00325(nox)

LRG:

LRHM_0312

LRL:	LC705_00311(nox)

LRA:	LRHK_319(nox)

LRO:	LOCK900_0301

LRC:	LOCK908_0315

LCHI:

KG086_01195

LZE:	KG087_01400

LHN:	LHUE1_001367

LPAH:

LACPH_000282

LSB:	LACSTY_000278

LMAE:

FGL90_01365

LROS:

LROSL1_2334

LHB:	D1010_01590

WDI:	H9L19_07180

XAK:	KIMC2_04430

XAP:

XA3_14520

CAC:	CA_C1027 CA_C2449

CAE:	SMB_G1045(fprA) SMB_G2484(fprA)

CAY:	CEA_G1039 CEA_G2463

CPE:	CPE0288 CPE1014

CPF:	CPF_0284 CPF_1269

CPR:	CPR_0275 CPR_1091

CTC:

CTC_01004

CTET:

BN906_01067

CNO:	NT01CX_1654

CBO:

CBO2619

CBA:

CLB_2561

CBH:

CLC_2492

CBY:

CLM_2982

CBL:

CLK_2005

CBB:

CLD_1946

CBI:

CLJ_B2849

CBN:

CbC4_0837

CBF:

CLI_2683

CBM:

CBF_2675

CBJ:	H04402_02712

CBE:	Cbei_4481 Cbei_4483

CBZ:	Cbs_4481 Cbs_4483

CBEI:

LF65_05014 LF65_05016

CKL:	CKL_3321(fprA)

CKR:

CKR_2929

CLJ:	CLJU_c21940

CCB:	Clocel_1666

CSR:	Cspa_c08450(fprA1) Cspa_c08470(fprA2)

CPAS:

Clopa_1032 Clopa_1671

CPAT:

CLPA_c10580(fprA2)

CPAE:

CPAST_c10580(fprA2)

CAH:	CAETHG_0291

CLT:	CM240_1207(fprA1)

CBV:	U729_2924(fprA2)

CSQ:

CSCA_1492

CLD:	CLSPO_c26930(fprA1)

CACE:

CACET_c35310

CCK:	Ccar_07240

CEU:	A7L45_15690

CFM:	BJL90_03480

CCHV:

BTM20_01910

CARG:

RSJ17_05515 RSJ17_11705

CDRK:

B9W14_11805

CIA:	BEN51_09430 BEN51_12645

CSEP:

CP523_06540 CP523_13435

CDY:	F3K33_22940 F3K33_22950

CCOH:

SAMEA4530647_0828(fprA1)

CGAS:

J1C67_07255 J1C67_12525

CLOS:

DMR38_11295

CCAA:

KQH81_02920

CMAH:

C1I91_21805 C1I91_27065

CRW:	CROST_015780(fprA1)

CFB:	CLFE_016500(fprA1)

CAUN:

CLAUR_013280(fprA1)

CGEL:

psyc5s11_38240

CTAG:

LL095_16140 LL095_19740

CINT:

HZF06_02400

CLOD:

C820_001542(norV)

ASF:

SFBM_0059

ASM:	MOUSESFB_0059

ASO:	SFBmNL_00074

ASB:	RATSFB_0060

HHW:	NCTC503_00298(fprA1_1) NCTC503_01057(fprA1_2)

CALE:

FDN13_04340

CRS:	FQB35_11305

CPRF:

K7H06_03520 K7H06_16880

SARJ:

HH195_05345

OCW:	OW730_00410 OW730_20300

EHA:	Ethha_1061

ACOL:

K5I23_16800

PETR:

QKW49_16275

PALM:

RBG61_01660

OKO:	Ruko_17660

CCEL:

CCDG5_0978(fprA2)

CAPR:

EQM14_01055

CFEM:

HCR03_15400

SWO:	Swol_2054 Swol_2363

SLP:

Slip_1536

DAI:	Desaci_0640

DAE:

Dtox_2294

ACAE:

HYG86_10775

CTHM:

CFE_0293

IBU:	IB211_02921

BPRM:

CL3_22630

BYL:	A4V09_16140

BHAN:

CGC63_03185

BLAU:

DQQ01_03010

BPRO:

PMF13cell1_01797(fprA1)

BLIQ:

INP51_00335

BLAR:

LC508_14805

BCOC:

BLCOC_08460(norV)

BHV:	BLHYD_05110(norV)

LACY:

A4V08_25210

CSO:

CLS_09940

ACHT:

bsdcttw_07790

RGN:	RGna_17470

CBOL:

CGC65_05545

ECLF:

QMP25_37820

SINT:

K5I26_04275

SCAG:

ACI3DM_07195

SCAZ:

ACI3DN_04645

QDO:	H9Q78_14125

WHJ:	H9Q79_12870

LBX:

lbkm_1425

CPRO:

CPRO_00260(fprA1)

ANV:	RBQ60_00160

CLE:	Clole_2026

CEW:	EKH84_12250

VGU:	HYG85_08030 HYG85_16400

VPY:	HZI73_04160 HZI73_20765

AHAD:

FRZ06_20305

CSPO:

QNI18_01720

PBIF:

KXZ80_03170 KXZ80_06615

PSOR:

RSJ16_03120 RSJ16_07995

CDF:	CD630_11570(norV) CD630_16230

PDC:	CDIF630_01304(fprA) CDIF630_01800(fprAnorR)

CDC:	CD196_1016(norV) CD196_1546

CDL:	CDR20291_0994(norV) CDR20291_1521

PDF:	CD630DERM_11570(norV) CD630DERM_16230

ROC:	HF520_02930

RIL:

CRIB_409

RHOM:

FRIFI_0500

PHX:	KGNDJEFE_01933(fprA1)

TEM:	JW646_07820 JW646_12860

TGC:	L0P85_00705 L0P85_14815

TMY:	TEMA_22400(fprA1) TEMA_32640(norV)

TPET:

TPHSE_03170(norV) TPHSE_33210(fprA1)

TEB:	T8CH_2350(fprA)

THYR:

P4S50_05435

IBR:	NQ514_11530

TTM:

Tthe_1029

TTO:	Thethe_00986

TXY:	Thexy_1693

TSH:

Tsac_1486

MAS:	Mahau_2344

HPRF:

HLPR_23730

CAZO:

G3A45_11855

TIS:	P3962_11580

SEDM:

RBQ61_13820

TUR:	AT726_01520 AT726_05065

TSG:	HLK68_03790 HLK68_14320

TBB:	J0J69_04240 J0J69_07985

TFC:	T23_14120 T23_19390

TUK:	VXM77_01250 VXM77_04600

CSC:

Csac_1982

ATE:

Athe_1435

COB:	COB47_1119

CHD:	Calhy_1301

COW:	Calow_1214

CKI:	Calkr_1420

CLC:	Calla_0805

CKN:	Calkro_1275

CCHA:

ELD05_06245

CDIA:

CaldiYA01_10840

CNAG:

OTJ99_001287

CMOR:

OTK00_001121

CDAN:

SOJ16_001392

ETT:	AZF37_06205

MYG:	ASO20_00625

MGJ:

MGM1_5040

TBK:	HF295_04280

OCY:	OSSY52_13870

FHO:	H9Q81_05250

CSOM:

MKD34_07280

» show all

Reference

1 [PMID:3082630]

Authors

Schmidt HL, Stocklein W, Danzer J, Kirch P, Limbach B

Title

Isolation and properties of an H2O-forming NADH oxidase from Streptococcus faecalis.

Journal

Eur J Biochem 156:149-55 (1986)
DOI:10.1111/j.1432-1033.1986.tb09560.x

Reference

2 [PMID:8254304]

Authors

Higuchi M, Shimada M, Yamamoto Y, Hayashi T, Koga T, Kamio Y

Title

Identification of two distinct NADH oxidases corresponding to H2O2-forming oxidase and H2O-forming oxidase induced in Streptococcus mutans.

Journal

J Gen Microbiol 139:2343-51 (1993)
DOI:10.1099/00221287-139-10-2343

Sequence

[smu:SMU_1117]

Reference

3 [PMID:8824824]

Authors

Matsumoto J, Higuchi M, Shimada M, Yamamoto Y, Kamio Y

Title

Molecular cloning and sequence analysis of the gene encoding the H2O-forming NADH oxidase from Streptococcus mutans.

Journal

Biosci Biotechnol Biochem 60:39-43 (1996)
DOI:10.1271/bbb.60.39

Sequence

[smu:SMU_1117]

Reference

4 [PMID:15014929]

Authors

Kawasaki S, Ishikura J, Chiba D, Nishino T, Niimura Y

Title

Purification and characterization of an H2O-forming NADH oxidase from Clostridium aminovalericum: existence of an oxygen-detoxifying enzyme in an obligate anaerobic bacteria.

Journal

Arch Microbiol 181:324-30 (2004)
DOI:10.1007/s00203-004-0659-3

Reference

5 [PMID:22418266]

Authors

Zhang YW, Tiwari MK, Gao H, Dhiman SS, Jeya M, Lee JK

Title

Cloning and characterization of a thermostable H2O-forming NADH oxidase from Lactobacillus rhamnosus.

Journal

Enzyme Microb Technol 50:255-62 (2012)
DOI:10.1016/j.enzmictec.2012.01.009

Sequence

[lrg:LRHM_0312]