KEGG ENZYME: 2.4.1.230

ENZYME: 2.4.1.230

Entry

EC 2.4.1.230                Enzyme

Name

kojibiose phosphorylase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

2-alpha-D-glucosyl-D-glucose:phosphate beta-D-glucosyltransferase

Reaction(IUBMB)

2-alpha-D-glucosyl-D-glucose + phosphate = D-glucose + beta-D-glucose 1-phosphate [RN:R07264]

Reaction(KEGG)

R07264

Substrate

2-alpha-D-glucosyl-D-glucose [CPD:C15548];
phosphate [CPD:C00009]

Product

D-glucose [CPD:C00031];
beta-D-glucose 1-phosphate [CPD:C00663]

Comment

The enzyme from Thermoanaerobacter brockii can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. The enzyme is inactive when dissaccharides with linkages other than alpha-1,2 linkages, such as sophorose, trehalose, neotrehalose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, gentiobiose, sucrose and lactose, are used as substrates.

History

EC 2.4.1.230 created 2003

Orthology

K10231

kojibiose phosphorylase

Genes

ECO:	b1316(ycjT)

ECJ:	JW1309(ycjT)

ECD:	ECDH10B_1433(ycjT)

EBW:	BWG_1148(ycjT)

ECOK:

ECMDS42_1114(ycjT)

ECOC:

C3026_07710

ECE:	Z2467(ycjT)

ECS:	ECs_1895(ycjT)

ECF:	ECH74115_1961

ETW:	ECSP_1841(ycjT)

ELX:	CDCO157_1811

EOJ:	ECO26_1884(ycjT)

EOH:	ECO103_1480(ycjT)

ECOO:

ECRM13514_1744(ycjT)

ECOH:

ECRM13516_1708(ycjT)

ESL:

O3K_13750

ESO:

O3O_11880

ESM:

O3M_13725

ECK:	EC55989_1480(ycjT)

EOK:	G2583_1663(ycjT)

ELR:	ECO55CA74_08105

ELH:

ETEC_1420

ECW:	EcE24377A_1527

EUN:	UMNK88_1653

ECP:

ECP_1368

ENA:	ECNA114_1506(ycjT)

ECOS:

EC958_1647(ycjT)

ECV:	APECO1_469(ycjT)

ECOA:

APECO78_10455

ECX:	EcHS_A1431

ECM:	EcSMS35_1806

ECY:

ECSE_1368

ECR:	ECIAI1_1341(ycjT)

ECQ:	ECED1_1524(ycjT)

ECT:	ECIAI39_1668(ycjT)

EOC:	CE10_1564(ycjT)

EBR:	ECB_01293(ycjT)

ECOB:

C3029_11030

EBL:	ECD_01293(ycjT)

EBE:	B21_01304(ycjT)

EBD:

ECBD_2301

ECI:	UTI89_C1587(ycjT)

EIH:	ECOK1_1531

ECZ:	ECS88_1458(ycjT)

ECC:	c1788(ycjT)

ELN:	NRG857_06755

ESE:

ECSF_1297

ECL:	EcolC_2309

EKF:	KO11_16230(ycjT)

EAB:	ECABU_c15990(ycjT)

EDH:	EcDH1_2330

EDJ:	ECDH1ME8569_1258(ycjT)

ELU:	UM146_10195

ELW:	ECW_m1412(ycjT)

ELL:	WFL_06885(ycjT)

ELC:	i14_1615(ycjT)

ELD:	i02_1615(ycjT)

ELP:	P12B_c1778

ELF:	LF82_2776(ycjT)

ECOL:

LY180_06700

ECOI:

ECOPMV1_01512(kojP)

ECOJ:

P423_07735

SES:	SARI_01291

SBG:

SBG_1540

SBZ:

A464_1761

SBV:	N643_07410

SFL:	SF1322(ycjT)

SFX:	S1405(ycjT)

SFV:	SFV_1332(ycjT)

SFE:	SFxv_1497(ycjT)

SFN:

SFy_1902

SFS:

SFyv_1959

SFT:	NCTC1_01402(ycjT)

SSN:	SSON_1824(ycjT)

SHQ:	A0259_10705

ENC:

ECL_01787

ENL:	A3UG_12340

ECLG:

EC036_23330

ECLE:

ECNIH2_12275

ECLN:

ECNIH4_10785

ECLI:

ECNIH5_10290

ECLX:

LI66_12015

ECLY:

LI62_13845

ECLZ:

LI64_11850

ECLO:

ENC_08770

EHM:	AB284_13350

EXF:	BFV63_12020

ECLA:

ECNIH3_11535

ECLC:

ECR091_11475

EAU:	DI57_06725

EKB:	BFV64_12220

ENO:	ECENHK_12175

EEC:	EcWSU1_02466(ycjT)

ELG:	BH714_08155

ECAN:

CWI88_10655

ERN:	BFV67_11705

ECHG:

FY206_13460

ESH:	C1N69_12350

EPT:	HWQ17_00885

ENT:	Ent638_2158

ENX:	NI40_012115

LNI:	CWR52_22740

EBG:	FAI37_16885

END:	A4308_16540

ENZ:	G0034_12280

ENS:	HWQ15_20685

ENK:	LOC22_00165

EHU:	D5067_0010540

EMOR:

L6Y89_11735

ENB:	ELK40_12670

EQU:	OM418_12075

EPU:	QVH39_12855

EDY:	F0320_11750

ESA:

ESA_01648

CSK:	ES15_1836(ycjT)

CSZ:	CSSP291_08040

CSJ:	CSK29544_02908

CCON:

AFK62_10410

CDM:	AFK67_10685

CSI:	P262_02625

CMJ:	AFK66_008695

CUI:	AFK65_10880

CMW:	AFK63_08025

CTU:	CTU_23090(ycjT)

CRO:

ROD_16931

CFD:	CFNIH1_18660

CBRA:

A6J81_05115

CFQ:	C2U38_11350

CSED:

JY391_10550

CAF:	AL524_14385

CFAR:

CI104_13935

CIR:	C2U53_22940

CIE:	AN232_17160

CITZ:

E4Z61_05840

CARS:

E1B03_14125

CIX:	M4I31_10935

CIB:	HF677_011175

CENS:

P2W74_11330

CITR:

GUC46_11130

CIQ:	FD428_10565

CIY:	HAP28_12790

LAX:	APT61_09725

LEH:	C3F35_21980

LEA:	GNG26_12625

LAZ:	A8A57_11575

LEW:	DAI21_14930

BUF:	D8682_03475

BAGE:

BADSM9389_20590

BFT:	MNO13_12020

BSEL:

RHD99_11230

AHN:	NCTC12129_02438(kojP)

ASUB:

NLZ15_11585

SGOE:

A8O29_012870

SCOL:

KFZ77_10095

SUPE:

P0H77_10270

FPUL:

ACBV55_10160

EBB:

F652_1714

RAH:	Rahaq_3913

RACE:

JHW33_13890

RAQ:	Rahaq2_4023

RAA:

Q7S_19900

RBON:

QNM34_19940

CARU:

P0E69_01015

NIG:	C1N62_00945

LPV:	HYN51_10765

VOW:	A9237_25500

VCY:	IX92_18550

VCT:	JV59_20490

PIE:	HU724_012425

GFU:	KM031_10265

TAER:

GT409_06700

SMON:

AWR27_12525

CTE:

CT0838

CPC:

Cpar_0865

CLZ:	BIU88_06910

CPH:	Cpha266_1194

CPB:	Cphamn1_1203

CLI:

Clim_0811

PVI:

Cvib_1015

PLT:

Plut_1264

PPH:

Ppha_1550

PAA:

Paes_0919

PROC:

Ptc2401_01014(kojP)

PROS:

CHL67_06640

PROI:

OO005_05040

BACF:

AM500_19315

BEO:

BEH_14395

OJD:	NP439_15375

HNZ:	P9989_04125

HSHI:

MUO14_17480

HAMY:

MUO15_09285

VNT:	OLD84_18865

BMUR:

ABE28_006470

PSYO:

PB01_10105

PSYB:

KD050_01480

NTM:	BTDUT50_05985

NNV:	QNH39_24090

MDG:	K8L98_04105

GAJ:	MY490_03125 MY490_18795

FHL:	OE105_10770

FAF:	OE104_14845

AFL:

Aflv_2025

AGN:	AFK25_04285

AAYD:

B379_02510

LMO:

lmo2833

LMN:	LM5578_0024

LMY:	LM5923_0024

LMT:	LMRG_01865

LMOC:

LMOSLCC5850_2843

LMOB:

BN419_3377

LMOD:

LMON_2852

LMOW:

AX10_08350

LMOQ:

LM6179_00253

LMR:	LMR479A_2971(ycjT)

LMF:	LMOf2365_2824

LMC:	Lm4b_02806

LMOG:

BN389_28150(ycjT)

LMP:

MUO_14235

LMOL:

LMOL312_2798

LMOZ:

LM1816_19790

LMOX:

AX24_12360

LMH:	LMHCC_2688

LMQ:	LMM7_2954(ycjT)

LML:	lmo4a_2899

LMG:	LMKG_01997

LMS:

LMLG_0198

LMJ:	LMOG_01656

LMW:	LMOSLCC2755_2854

LMX:	LMOSLCC2372_2913

LMZ:	LMOSLCC2482_2851

LMON:

LMOSLCC2376_2732

LMOS:

LMOSLCC7179_2803

LMOO:

LMOSLCC2378_2851

LMOY:

LMOSLCC2479_2912

LMOT:

LMOSLCC2540_2884

LMOA:

LMOATCC19117_2844

LMOK:

CQ02_14520

LMV:	Y193_01420

LIN:

lin2966

LWE:

lwe2763

LIV:

LIV_2766

LII:	JL52_00165

LIW:	AX25_00175

LIA:	JL58_00100

LIO:	JL53_00100

LWI:	UE46_15145 UE46_16080

LMAR:

LAX80_013875

LNW:	OTR81_06250 OTR81_11700

EAT:	EAT1b_1273

EAP:	KB235_04175

EMM:	PTI97_11815

PAEF:

R50345_24670

PIH:	UB51_05845

PRI:	PRIO_1698 PRIO_1720

PKB:	B4V02_12625

PVO:	PVOR_04088

PLUT:

EI981_05360 EI981_07980 EI981_22665

PTHI:

NDS46_23175

PMAH:

PTQ21_00830

PMAE:

LMZ02_03665

PPAB:

KET34_29820

PGLU:

A3958_11205

PEZZ:

HW560_10165

PANA:

BBH88_16945

PDG:	BCM40_01195

PMAR:

B0X71_18105

PLAY:

DNR44_000410

PSHX:

QWY21_04650 QWY21_17550

SPAE:

E2C16_10245

SURL:

BI350_14295

LLM:

llmg_0487

LLN:	LLNZ_02520

LLW:

kw2_0467

LACT:

D7I46_12815

LACK:

FLP15_05165

LACN:

QJV49_10010

LHI:	JP39_10380 JP39_10410

LCT:	BI355_1969 BI355_1970

LALI:

LA20249_09495

LFM:	LF20184_01575

LZH:	D1B17_11275 D1B17_11290

LFT:	FG051_01165

CPAB:

G6534_00110

OEN:	DSM07_06795

EFC:	EFAU004_02822

EFAU:

EFAU085_02880

EFU:	HMPREF0351_12656

EFM:

M7W_2671

EFT:	M395_12850

EGA:	AL523_01285

EAV:	EH197_11195

EIE:	ENLAB_10770(mapA_2)

ENI:	CXM95_14475

VFV:	K5K99_02860

HHW:	NCTC503_02668(kojP)

FSA:	C5Q98_05670

CLF:	GJQ69_08400

AIM:	PYS61_03310

ANQ:	PYS63_01640

BPRS:

CK3_32510

RIM:

ROI_37010

CCOM:

I6K69_12820

BLIQ:

INP51_11335

BLAR:

LC508_01820 LC508_20485

BCOC:

BLCOC_34910(ycjT)

BHV:	BLHYD_32840(ycjT)

LACY:

A4V08_07380

CSH:	Closa_0063 Closa_0870

LGG:	QJR73_09185 QJR73_11140

SINT:

K5I26_05850

SCAZ:

ACI3DN_08775

HHJ:	NQ487_03030 NQ487_18815

CEW:	EKH84_03885

VPY:	HZI73_07615

TTE:	TTE0798(Ath1)

TEX:	Teth514_2202

THX:

Thet_0733

TPD:	Teth39_1521

TIT:

Thit_0754

TMT:	Tmath_0796

TBO:	Thebr_1557

TWI:	Thewi_0897

TKI:	TKV_c08300

THEZ:

OEI98_000902

TXY:	Thexy_1672

TSH:

Tsac_1508

HOR:	Hore_00420

HPK:	Hprae_0387

HHL:	Halha_1832

AFT:	BBF96_14310

CSC:

Csac_0439

ATE:

Athe_0398

COB:	COB47_0356

CHD:	Calhy_2252

CKI:	Calkr_0343

CLC:	Calla_2015

CKN:	Calkro_2237

CCHA:

ELD05_02050

CDIA:

CaldiYA01_20380(kojP)

CNAG:

OTJ99_000331

CDAN:

SOJ16_000349

MCD:

MCRO_0717

MANS:

FRW55_02140

MBRA:

NMG68_03445

EFR:	EFREU_v1c05930(mapA)

MMO:

MMOB3950

MHR:

MHR_0186

MHH:

MYM_0194

MHM:

SRH_01030

MHS:

MOS_212

MHV:

Q453_0211

MDS:	MDIS_02910

MNU:	NCTC10166_00383(kojP)

MOE:	NCTC10151_00452(kojP)

MLAG:

QEG99_00485

MGB:	VO56_02745

MGLY:

NCTC10194_00675(kojP)

MCOU:

NCTC10179_00389(kojP)

MPU:	MYPU_6340(MYPU_6340)

MSY:

MS53_0107

MSO:	VY93_00585

MFR:

MFE_05890

MFM:	MfeM64YM_0704

MFP:

MBIO_0859

MCY:	MCYN_0087(MCYN0087)

MCAS:

AAW50_02485

MCK:	AXW82_02390

MPUL:

BLA55_00020 BLA55_03735 BLA55_04050

MBOH:

CO229_00660

MANI:

DP067_01365

MFEL:

JPM2_5110

MGAL:

NCTC10186_00807(kojP)

MCIT:

NCTC10181_00942(kojP)

MCAV:

NPA07_02045

MGF:

MGF_3567

MCAG:

MCAV_05410

MMAT:

MMAGJ_02490

ASD:

AS9A_2798

TSD:	MTP03_18670

ACIT:

HPK19_14375 HPK19_21395

MPH:	MLP_44690(kojP)

PHH:	AFB00_27045

BLF:

BLIF_0278

BBV:	HMPREF9228_1760(kojP)

BBRU:

Bbr_1693(kojP)

BBRN:

B2258_1711

BBRS:

BS27_1683(kojP)

GVG:	HMPREF0421_21250(kojP)

GVH:	HMPREF9231_0272(kojP)

CMP:	Cha6605_1181 Cha6605_6063

CYN:	Cyan7425_3917

CEP:	Cri9333_1325

LEN:	LEP3755_51020 LEP3755_55090

LBO:	LBWT_4840 LBWT_9600

KOV:	K9N68_24665(pgmB)

LSC:	KIK02_02005(pgmB)

LSK:	J5X98_09615(pgmB)

PGX:	OA858_23895(pgmB)

GLP:	Glo7428_0521 Glo7428_4259

GDU:	P0S91_09635(pgmB)

CYJ:	Cyan7822_4895

OXY:	HCG48_11840(pgmB)

OAC:	Oscil6304_2195

MIC:	Mic7113_3750

MPRO:

BJP34_23515

GEI:	GEI7407_2145

ANA:	all1058 all4989

NPU:	Npun_F4952

NOS:	Nos7107_1513

NOP:	Nos7524_2895 Nos7524_4006

NON:	NOS3756_14110

NFL:	COO91_03953

NOE:	CLI64_26940(pgmB)

NSH:

GXM_06418

NED:	HUN01_32070 HUN01_33615(pgmB)

NCW:	NIES2107_19170 NIES2107_70290

NOST:

GJB62_12680(pgmB)

NOSU:

JYQ62_04045(pgmB)

NOSQ:

PQG02_04800 PQG02_16740(pgmB)

AVA:	Ava_2279 Ava_3752

NAZ:

Aazo_0214

ANB:	ANA_C11945

ACY:	Anacy_4233

AWA:	AA650_01550

ANN:	EH233_15750

CSG:	Cylst_1833

RSIN:

B6N60_04008

HBQ:	QI031_00835(pgmB)

NSP:	BMF81_03401(kojP)

NSPH:

BDGGKGIB_01183(kojP)

AEE:	IM676_00375(pgmB)

CALO:

Cal7507_2213 Cal7507_4446

CALT:

Cal6303_2135

CALN:

NIES2098_00650 NIES2098_43080

CALS:

NIES3974_05250

RIV:	Riv7116_2936 Riv7116_3507 Riv7116_6086

MDP:	NIES3275_12820 NIES3275_45600

FIS:	FIS3754_09200 FIS3754_45460

DOU:	BMF77_00524(kojP)

DFS:	HGD76_13410(pgmB)

DHT:	NG743_15540(pgmB)

DCM:	NIES806_24870

DOT:	EZY12_08570(pgmB)

DOZ:	HEP80_15510(pgmB)

DOC:	HEQ26_01915(pgmB)

CRK:	L3I90_15310(pgmB)

STOQ:

K2F26_13345(pgmB)

SKV:	NIES73_25550

RCS:	NIES932_23880

OCF:	WJM97_03080(pgmB)

TOQ:	HCG51_14155(pgmB) HCG51_32300(pgmB)

TTQ:	NIES37_36340

ALAX:

NIES50_50250

SCYT:

SAMD_09870 SAMD_74250

SCYS:

NIES4073_24420 NIES4073_80670

BSEN:

DP114_29220(pgmB)

BOX:	DP113_29415(pgmB)

NCN:	BZZ01_08905 BZZ01_19490

PLP:	Ple7327_2002

CAU:

Caur_2019

CHL:	Chy400_2178

CAG:

Cagg_2264

ATM:

ANT_24940

CAP:	CLDAP_31030

PBF:	CFX0092_B0628

KBS:	EPA93_19085

KBB:	ccbrp13_67430

TTA:	Theth_1552

TFE:	JYK00_05610

FPE:	Ferpe_1597

FRR:	IB67_03615

FNG:	JM64_05320

PMO:

Pmob_0140

MAEO:

JRV97_00560

OCS:	OF820_09475

CSOM:

MKD34_09940

LGO:	JCM16774_1901

STR:	Sterm_1530

PHO:	PH0746(PH0746)

PYS:

Py04_1502

PYC:	TQ32_08780

TBA:	TERMP_00275

TTD:	A3L14_04400

TRL:	A3L10_03735

PPAC:

PAP_08195

TPE:

Tpen_1155

THB:	N186_07480

» show all

Reference

Authors

Chaen H, Yamamoto T, Nishimoto T, Nakada T, Fukuda S, Sugimoto T, Kurimoto M, Tsujisaka Y.

Title

Purification and characterization of a novel phosphorylase, kojibiose phosphorylase, from Thermoanaerobium brockii.

Journal

J Appl Glycosci 46:423-429 (1999)

Reference

2 [PMID:16233080]

Authors

Chaen H, Nishimoto T, Nakada T, Fukuda S, Kurimoto M, Tsujisaka Y.

Title

Enzymatic synthesis of kojioligosaccharides using kojibiose phosphorylase.

Journal

J Biosci Bioeng 92:177-82 (2001)
DOI:10.1016/S1389-1723(01)80221-8

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.230

IUBMB Enzyme Nomenclature:

2.4.1.230

ExPASy - ENZYME nomenclature database:

2.4.1.230

BRENDA, the Enzyme Database:

2.4.1.230

CAS:	206566-36-1

LinkDB

DBGET integrated database retrieval system