KEGG   ENZYME: 2.7.1.238
Entry
EC 2.7.1.238                Enzyme                                 
Name
phenol phosphorylase;
phenylphosphate synthase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
Sysname
ATP:phenol phosphotransferase (AMP-forming)
Reaction(IUBMB)
ATP + phenol + H2O = AMP + phenyl phosphate + phosphate [RN:R05625]
Reaction(KEGG)
R05625
Substrate
ATP [CPD:C00002];
phenol [CPD:C00146];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
phenyl phosphate [CPD:C02734];
phosphate [CPD:C00009]
Comment
The enzyme, characterized from the bacterium Thauera aromatica, catalyses the first step in an anaerobic phenol degradation pathway. The enzyme, composed of three subunits, transfers the beta-phosphoryl from ATP to phenol, forming phenyl phosphate, AMP, and phosphate [1]. During catalysis a diphosphoryl group is transferred from ATP to a histidine residue in one of the enzyme's subunits, from which phosphate is cleaved to render the reaction unidirectional. The remaining histidine phosphate subsequently serves as the actual phosphorylation agent [2].
History
EC 2.7.1.238 created 2022
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K25936  phenol phosphorylase subunit alpha
K25937  phenol phosphorylase subunit beta
K25938  phenol phosphorylase subunit delta gamma
Genes
EBA: ebA3143(ppsB) ebA3144(ppsA) ebD7(ppsC)
ABRE: pbN1_19200(ppsC) pbN1_19210(ppsB) pbN1_19220(ppsA)
APET: ToN1_08850(ppsA) ToN1_08860(ppsB) ToN1_08870(ppsC)
AZA: AZKH_p0129(ppsA) AZKH_p0130(ppsB) AZKH_p0131(ppsC)
AZI: AzCIB_2684 AzCIB_2685 AzCIB_2686
AZD: CDA09_14585 CDA09_14590 CDA09_14595
TAK: Tharo_2774 Tharo_2775 Tharo_2776
DDZ: DSYM_29710 DSYM_29720 DSYM_29730
ACOB: P0Y56_06840 P0Y56_06845
GME: Gmet_2100 Gmet_2101 Gmet_2104
GEO: Geob_0138 Geob_0141 Geob_0142
DTO: TOL2_C15950(ppsA) TOL2_C15960(ppsB) TOL2_C16020(ppsC)
DTI: Desti_0829 Desti_0830 Desti_0834
DBR: Deba_2753
DMP: FAK_08810 FAK_08850 FAK_08860
MKY: IWGMT90018_46640
MYW: BVC93_12820 BVC93_12825
RHOJ: JVH1_20780 JVH1_20785
RHJS: OYT95_39565 OYT95_39570
SGRF: SGFS_009550 SGFS_009560
MLIY: RYJ27_00250
CMUA: P8192_10995 P8192_11000
NOCA: ncot_19140
BBRG: U6N30_06500
BMON: ABDB74_14545
ARHD: VSH64_19055
AMYD: K1T34_12590
PSDS: K1T35_18450 K1T35_18455
ACTY: OG774_07835 OG774_07930 OG774_21235
SBAE: DSM104329_00598(cmdD_1) DSM104329_02332 DSM104329_02333(ppsA_2)
 » show all
Reference
1  [PMID:15547277]
  Authors
Schmeling S, Narmandakh A, Schmitt O, Gad'on N, Schuhle K, Fuchs G.
  Title
Phenylphosphate synthase: a new phosphotransferase catalyzing the first step in anaerobic phenol metabolism in Thauera aromatica.
  Journal
J Bacteriol 186:8044-57 (2004)
DOI:10.1128/JB.186.23.8044-8057.2004
  Sequence
[tak:Tharo_2774 Tharo_2775 Tharo_2776]
Reference
2  [PMID:16980461]
  Authors
Narmandakh A, Gad'on N, Drepper F, Knapp B, Haehnel W, Fuchs G.
  Title
Phosphorylation of phenol by phenylphosphate synthase: role of histidine phosphate in catalysis.
  Journal
J Bacteriol 188:7815-22 (2006)
DOI:10.1128/JB.00785-06
  Sequence
[tak:Tharo_2774 Tharo_2775 Tharo_2776]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.238
IUBMB Enzyme Nomenclature: 2.7.1.238
ExPASy - ENZYME nomenclature database: 2.7.1.238
BRENDA, the Enzyme Database: 2.7.1.238
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