KEGG ENZYME: 4.3.3.9

ENZYME: 4.3.3.9

Entry

EC 4.3.3.9                  Enzyme

Name

indigoidine synthase;
bspA (gene name)

Class

Lyases;
Carbon-nitrogen lyases;
Amine-lyases

Sysname

L-glutamine oxidoreductase/cyclase (3-amino-1,5-dihydropyridine-2,6-dione-forming)

Reaction(IUBMB)

2 ATP + 2 L-glutamine + O2 + 2 FMN = 2 AMP + 2 diphosphate + indigoidine + 2 H2O + 2 FMNH2 (overall reaction) [RN:R13355];
(1a) 2 ATP + 2 L-glutamine + 2 FMN = 2 AMP + 2 diphosphate + 2 3-amino-1,5-dihydropyridine-2,6-dione + FMNH2 [RN:R13353];
(1b) 2 3-amino-1,5-dihydropyridine-2,6-dione + O2 = indigoidine + 2 H2O (spontaneous) [RN:R13354]

Reaction(KEGG)

R13353 R13354 R13355

Substrate

ATP [CPD:C00002];
L-glutamine [CPD:C00064];
O2 [CPD:C00007];
FMN [CPD:C00061];
3-amino-1,5-dihydropyridine-2,6-dione [CPD:C22943]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
indigoidine [CPD:C22944];
H2O [CPD:C00001];
FMNH2 [CPD:C01847];
3-amino-1,5-dihydropyridine-2,6-dione [CPD:C22943]

Comment

The enzyme, found in a number of bacterial strains, is a non-ribosomal peptide synthase (NRPS). The enzyme forms 3-amino-1,5-dihydropyridine-2,6-dione, which undergoes spontaneous oxidation to form the blue pigment indigoidine.

History

EC 4.3.3.9 created 2024

Orthology

K27871

indigoidine synthase

Genes

SPE:

Spro_1698

SQU:	E4343_07645

DDD:	Dda3937_00972(indC)

DZE:	Dd1591_0073

DDC:	Dd586_0084

DZC:	W909_00340

DSO:	A4U42_09535

CED:	LH89_11810

DFN:	CVE23_00465

DDQ:

DDI_4393

DAQ:	DAQ1742_04343(indC)

DIC:	Dpoa569_0003841

ETP:	LU633_16190

PLU:

plu2186

PLUM:

A4R40_10965

PAY:

PAU_02362

PTT:	VY86_20065

XPO:

XPG1_0720

VNI:	VIBNI_B0265(bpsA)

VSR:	Vspart_04306(tycC_7)

VRU:	RND59_00295

ACV:	AMD27_12430

PBW:	D172_001470

PRR:	AT705_17200

PNG:	PNIG_a0231(ofaC)

PAEW:

KIH87_06045

MMAA:

FR932_08020

BGD:	bgla_2g17770

PBH:	AAW51_5194

RJG:	CCGE525_37390

RTU:	PR017_21845

ARA:	Arad_12458

LEJ:	ETW24_21400

LAQU:

R2C4_02970

LCAE:

K3721_19825

AVM:	JQX13_29175

PIJ:	QEJ31_09450

CAR:	CAQ58242.1

CGV:	CGLAU_10015(tycC)

CSTR:

CBE89_13190

WHR:	OG579_02790

SALB:

XNR_5634

SFI:	SFUL_2479 SFUL_5936

SCYE:

R2B67_31530

SVE:

SVEN_4086

SDV:	BN159_7970(indC)

SVT:	SVTN_21155

STRE:

GZL_01654

SCZ:	ABE83_23580

SRN:	A4G23_04733(ppsD)

SNR:	SNOUR_41230

SPLU:

LK06_027050

SLAU:

SLA_4174

SLX:	SLAV_40115(tycC5)

SGD:	ELQ87_09885

SSPO:

DDQ41_19505

SCAV:

CVT27_10965

SGX:	H4W23_22610

SBRO:

GQF42_31590

SHAR:

HUT13_01155

SFB:	CP974_25355

SROI:

IAG44_11750

SMAO:

CAG99_14340

SGOB:

test1122_13725

STAA:

LDH80_18160

SYUN:

MOV08_06930

SHAU:

K9S39_05015

SCOA:

QU709_03980

SCAY:

PYS65_05785

KBU:	Q4V64_07135

KAB:	B7C62_03605

CMH:	VO01_05770

CUY:	I8920_00995

RSA:	RSal33209_1837

SATK:

SA2016_3652

BLUT:

EW640_11710

AER:	AERYTH_13670

NOA:	BKM31_11375

MHAI:

OHB01_07065

AOI:

AORI_6000

AJA:	AJAP_09435

AMYB:

BKN51_23530

AORI:

SD37_29415

ACYN:

ORV05_18235

AMYE:

MJQ72_29340

LED:	BBK82_06850

UME:	RM788_06215

MICH:

FJK98_13995

PSUU:

Psuf_005290

DROS:

Drose_28380

NAV:	JQS30_13885

NOSU:

JYQ62_14450

» show all

Reference

1 [PMID:11790734]

Authors

Reverchon S, Rouanet C, Expert D, Nasser W.

Title

Characterization of indigoidine biosynthetic genes in Erwinia chrysanthemi and role of this blue pigment in pathogenicity.

Journal

J Bacteriol 184:654-65 (2002)
DOI:10.1128/JB.184.3.654-665.2002

Sequence

[ag:CAB87990]

Reference

2 [PMID:17237222]

Authors

Takahashi H, Kumagai T, Kitani K, Mori M, Matoba Y, Sugiyama M.

Title

Cloning and characterization of a Streptomyces single module type non-ribosomal peptide synthetase catalyzing a blue pigment synthesis.

Journal

J Biol Chem 282:9073-81 (2007)
DOI:10.1074/jbc.M611319200

Sequence

[ag:BAE93896]

Reference

3 [PMID:23051833]

Authors

Walsh CT, Wencewicz TA.

Title

Flavoenzymes: versatile catalysts in biosynthetic pathways.

Journal

Nat Prod Rep 30:175-200 (2013)
DOI:10.1039/c2np20069d

Other DBs

ExplorEnz - The Enzyme Database:

4.3.3.9

IUBMB Enzyme Nomenclature:

4.3.3.9

ExPASy - ENZYME nomenclature database:

4.3.3.9

BRENDA, the Enzyme Database:

4.3.3.9

LinkDB

DBGET integrated database retrieval system