AR043193A1 - SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD - Google Patents
SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHODInfo
- Publication number
- AR043193A1 AR043193A1 ARP040100475A AR043193A1 AR 043193 A1 AR043193 A1 AR 043193A1 AR P040100475 A ARP040100475 A AR P040100475A AR 043193 A1 AR043193 A1 AR 043193A1
- Authority
- AR
- Argentina
- Prior art keywords
- sperm
- semen
- sex
- density gradient
- sexed semen
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Proceso de selección del sexo de espermatozoides mamíferos y método de control de calidad de dosis de sémen sexado congelado. Dicho proceso permite producir por centrifugación en gradiente de densidad, en empresas especializadas en la producción y comercialización de sémen congelado, dosis de sémen enriquecidas con espermatozoides portadores del cromosoma X o Y, sin comprometer la capacidad fecundativa, de estos espermatozoides. Dosis de espermatozoides que contienen ambos espermatozoides X e Y pueden ser separadas para producir subpoblaciones de espermatozoides enriquecidas con portadores del cromosoma X o Y, las cuales son sustancialmente puras con relación al espermatozoide deseado y sustancialmente libres el otro tipo de espermatozoide Reivindicación 1: Proceso de selección del sexo de espermatozoides mamíferos partiendo de la obtención del sémen in natura de reproductores según procedimientos de rutina, caracterizado por comprender la siguientes etapas: a) centrifugación en gradiente de densidad compuesto de medio de cultura químicamente definido para preservar la estabilidad e integridad de la membrana de los espermatozoides; b) utilización de temperaturas de 6 a 22sC, que preservan la viabilidad espermática; c) estabilización del pH en la banda fisiológica para cada especie, preferiblemente alrededor de 7,4; d) asociación a la presencia e sustancias que preserven la integridad espermática, utilizando preferiblemente glucosa, que evita la capacitación y reacción acrosómica precoces, especialmente en bovinos; y e) congelación de los espermatozoides luego de centrifugación en gradiente de densidad en diluidores propios para este propósito, tales como el uso de Tris-yema de huevo, para la utilización en la producción in vitro de embriones (PIV) o en la inseminación artificial (IA) convencional (nacimientos preferiblemente del sexo femenino o masculino o la realizadas después de tratamiento hormonal de las hembras para producir la sincronización del cío e/ou múltiples ovulaciones, cosecha de los embriones ( en su mayoría del sexo femenino o masculino) y posterior transferencia para hembras receptoras. Reivindicación 9: Sémen sexado según cualesquiera de las reivindicaciones anteriores, caracterizado por contener subpoblaciones de espermatozoides enriquecidas con portadores del cromosoma X o Y y ser sometido a las siguientes pruebas: control de calidad e las dosis de sémen que contienen espermatozoides centrifugados en gradiente de densidad, neque dicho protocolo de control de la calidad de fertilidad de los espermatozoides sometidos a sexaje es compuesto por: a) evaluación de motilidad y vigor y prueba de término resistencia (TTR); b) coloración específica para evaluar la integridad de la membrana de los espermatozoides; c) producción in vitro de embriones ; y d) inseminación artificial a través del método comercial. Reivindicación 10: Método de inseminación artificial, caracterizado por utilizar el sémen sexado según la reivindicación 9. Reivindicación 11: Método de producción in vitro de embriones, caracterizado por utilizar sémen sexado según la reivindicación 9.Selection process of mammalian sperm sex and method of dose quality control of frozen sexed semen. This process allows to produce by centrifugation in density gradient, in companies specialized in the production and commercialization of frozen semen, doses of semen enriched with sperm carrying the X or Y chromosome, without compromising the fertilization capacity of these sperm. Dose of sperm containing both X and Y sperm can be separated to produce subpopulations of sperm enriched with carriers of the X or Y chromosome, which are substantially pure relative to the desired sperm and substantially free of the other type of sperm Claim 1: Process of Selection of the sex of mammalian sperm based on the obtaining of the semen in natura from breeders according to routine procedures, characterized by comprising the following stages: a) density gradient centrifugation composed of chemically defined culture medium to preserve the stability and integrity of the sperm membrane; b) use of temperatures from 6 to 22sC, which preserve sperm viability; c) pH stabilization in the physiological band for each species, preferably around 7.4; d) association with the presence and substances that preserve sperm integrity, preferably using glucose, which prevents early training and acrosomic reaction, especially in cattle; and e) freezing of sperm after density gradient centrifugation in own diluents for this purpose, such as the use of egg Tris-yolk, for use in the in vitro production of embryos (PIV) or artificial insemination ( AI) Conventional (preferably female or male births or those made after hormonal treatment of females to produce synchronization of the spine and / or multiple ovulations, embryo harvest (mostly female or male) and subsequent transfer for recipient females Claim 9: Sexed semen according to any of the preceding claims, characterized by containing subpopulations of sperm enriched with carriers of the X or Y chromosome and being subjected to the following tests: quality control and semen doses containing centrifuged sperm in density gradient, neque said control protocol of the fertility quality of sperm subjected to sexing is composed of: a) evaluation of motility and vigor and endurance resistance test (TTR); b) specific coloration to assess the integrity of the sperm membrane; c) in vitro production of embryos; and d) artificial insemination through the commercial method. Claim 10: Artificial insemination method, characterized by using the sexed semen according to claim 9. Claim 11: Method of in vitro production of embryos, characterized by using sexed semen according to claim 9.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0300604A BR0300604A (en) | 2003-02-17 | 2003-02-17 | Sex selection process of mammalian sperm and quality control method of frozen sexed semen doses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR043193A1 true AR043193A1 (en) | 2005-07-20 |
Family
ID=32854956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040100475 AR043193A1 (en) | 2003-02-17 | 2004-02-17 | SELECTION PROCESS OF THE MAMIFER SpermATOZOID SEX AND FROZEN SEXED SEMEN DOSE QUALITY QUALITY CONTROL METHOD |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR043193A1 (en) |
| BR (1) | BR0300604A (en) |
| WO (1) | WO2004072220A2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118389491A (en) * | 2024-06-27 | 2024-07-26 | 成都棱镜泰克生物科技有限公司 | Quality control product for sperm DNA fragmentation detection and preparation method thereof |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050114915A1 (en) * | 2003-11-26 | 2005-05-26 | Vicam L.P. | Method for sex biasing spermatozoa |
| WO2008088601A2 (en) * | 2007-01-16 | 2008-07-24 | Texas Tech University System | Method and apparatus for gender selection based on ph |
| ITTO20100651A1 (en) | 2010-07-28 | 2012-01-29 | Ist Sperimentale It Lazzaro Spallanza | PROCEDURE AND EQUIPMENT FOR THE CHARACTERIZATION AND SEPARATION OF SPERMFERCEE WITH SUSPENDED LEVER MICROMETRIC SENSORS |
| CN104962513A (en) * | 2015-06-30 | 2015-10-07 | 阜阳师范学院 | Fox X sperm separating method |
| WO2019191682A1 (en) * | 2018-03-30 | 2019-10-03 | Texas Tech University System | System and method for non-invasive embryo sexing |
| CN109370981A (en) * | 2018-11-07 | 2019-02-22 | 临泉县欣达生态羊业有限公司 | The X Gender preselection of Anhui White Goats |
| CN109355250A (en) * | 2018-11-07 | 2019-02-19 | 临泉县欣达生态羊业有限公司 | The y sperm separation method of Anhui White Goats |
| EP4492061A1 (en) | 2023-07-11 | 2025-01-15 | Universidade de Trás-os-Montes e Alto Douro | Sex-specific sperm membrane proteins for sperm sexing |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3894529A (en) * | 1969-04-10 | 1975-07-15 | Bio Controls Inc | Method and means for controlling the sex of mammalian offspring and product therefor |
| SE0004777D0 (en) * | 2000-12-22 | 2000-12-22 | Amersham Pharm Biotech Ab | Separation of X and Y sperm cells |
-
2003
- 2003-02-17 BR BR0300604A patent/BR0300604A/en not_active Application Discontinuation
-
2004
- 2004-02-11 WO PCT/BR2004/000009 patent/WO2004072220A2/en active Search and Examination
- 2004-02-17 AR ARP040100475 patent/AR043193A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118389491A (en) * | 2024-06-27 | 2024-07-26 | 成都棱镜泰克生物科技有限公司 | Quality control product for sperm DNA fragmentation detection and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| BR0300604A (en) | 2004-11-03 |
| WO2004072220A3 (en) | 2004-10-07 |
| WO2004072220A2 (en) | 2004-08-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |
