BR9811396A - Subtilase enzyme variant having improved detergent washing performance, isolated DNA sequence, expression vector, microbial host cell, processes for producing a variant and for identifying a protease variant, composition, and, use of a subtilase variant - Google Patents

Subtilase enzyme variant having improved detergent washing performance, isolated DNA sequence, expression vector, microbial host cell, processes for producing a variant and for identifying a protease variant, composition, and, use of a subtilase variant

Info

Publication number
BR9811396A
BR9811396A BR9811396-8A BR9811396A BR9811396A BR 9811396 A BR9811396 A BR 9811396A BR 9811396 A BR9811396 A BR 9811396A BR 9811396 A BR9811396 A BR 9811396A
Authority
BR
Brazil
Prior art keywords
variant
subtilase
washing performance
processes
expression vector
Prior art date
Application number
BR9811396-8A
Other languages
Portuguese (pt)
Inventor
Peter Kamp Hansen
Peter Bauditz
Frank Mikkelsen
Kim Vilbour Andersen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9811396A publication Critical patent/BR9811396A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"VARIANTE DE ENZIMA SUBTILASE TENDO MELHORADO DESEMPENHO DE LAVAGEM EM DETERGENTES, SEQUêNCIA DNA ISOLADA, VETOR DE EXPRESSãO, CéLULA HOSPEDEIRA MICROBIANA, PROCESSOS PARA PRODUZIR UMA VARIANTE E PARA IDENTIFICAR UMA VARIANTE DE PROTEASE, COMPOSIçãO, E, USO DE UMA VARIANTE DE SUBTILASE". Enzimas produzidas por mutação dos genes para várias subtilases e expressando os genes mudados em hospedeiros apropriados são apresentadas. As enzimas demonstram melhorado desempenho de lavagem em qualquer detergente em comparação com suas enzimas originárias de tipo selvagem."SUBTYLASE ENZYM VARIANT HAVING IMPROVED WASHING PERFORMANCE IN DETERGENTS, ISOLATED DNA SEQUENCE, EXPRESSION VECTOR, MICROBIAL HOSTING CELL, PROCESSES TO PRODUCE A VARIANT AND TO IDENTIFY A PROTEIN, VARIANT, AND COMPARISON OF VARIANT, AND COMPOUND. Enzymes produced by mutating the genes for various subtilases and expressing the changed genes in appropriate hosts are presented. The enzymes demonstrate improved washing performance in any detergent compared to their wild-type enzymes.

BR9811396-8A 1997-08-29 1998-08-19 Subtilase enzyme variant having improved detergent washing performance, isolated DNA sequence, expression vector, microbial host cell, processes for producing a variant and for identifying a protease variant, composition, and, use of a subtilase variant BR9811396A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK98897 1997-08-29
PCT/DK1998/000361 WO1999011770A1 (en) 1997-08-29 1998-08-19 Protease variants and compositions

Publications (1)

Publication Number Publication Date
BR9811396A true BR9811396A (en) 2000-08-22

Family

ID=8099753

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811396-8A BR9811396A (en) 1997-08-29 1998-08-19 Subtilase enzyme variant having improved detergent washing performance, isolated DNA sequence, expression vector, microbial host cell, processes for producing a variant and for identifying a protease variant, composition, and, use of a subtilase variant

Country Status (8)

Country Link
EP (1) EP1007646A1 (en)
JP (1) JP2001514847A (en)
KR (1) KR20010023448A (en)
CN (1) CN1272881A (en)
AU (1) AU9061898A (en)
BR (1) BR9811396A (en)
CA (1) CA2301785A1 (en)
WO (1) WO1999011770A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002500019A (en) 1997-12-24 2002-01-08 ジェネンコア インターナショナル インコーポレーテッド Improved analytical method for preferred enzymes and / or preferred detergent compositions
JP5563180B2 (en) * 1999-05-20 2014-07-30 ノボザイムス アクティーゼルスカブ Subtilase enzymes of the I-S1 and I-S2 subgroups having at least one additional amino acid residue between positions 97 and 98
US7491362B1 (en) 2008-01-28 2009-02-17 Ecolab Inc. Multiple enzyme cleaner for surgical instruments and endoscopes
JP2011524166A (en) * 2008-06-06 2011-09-01 ダニスコ・ユーエス・インク Compositions and methods comprising mutant microbial proteases

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
ES2227508T3 (en) * 1989-06-26 2005-04-01 Unilever N.V. ENZYMATIC DETERGENT COMPOSITIONS.
CA2139928C (en) * 1992-07-17 2005-09-06 Leonardus Johannes Sofie Marie Mulleners High alkaline serine proteases
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis

Also Published As

Publication number Publication date
KR20010023448A (en) 2001-03-26
EP1007646A1 (en) 2000-06-14
JP2001514847A (en) 2001-09-18
AU9061898A (en) 1999-03-22
CA2301785A1 (en) 1999-03-11
CN1272881A (en) 2000-11-08
WO1999011770A1 (en) 1999-03-11

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Legal Events

Date Code Title Description
PC Transfer

Free format text: NOVOZYMES A/S (DK)

B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A, 7A, 8A E 9A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1909 DE 07/08/2007.