BR9911396A - Estabilização de enzima por meio de tensoativos catiÈnicos - Google Patents

Estabilização de enzima por meio de tensoativos catiÈnicos

Info

Publication number
BR9911396A
BR9911396A BR9911396-1A BR9911396A BR9911396A BR 9911396 A BR9911396 A BR 9911396A BR 9911396 A BR9911396 A BR 9911396A BR 9911396 A BR9911396 A BR 9911396A
Authority
BR
Brazil
Prior art keywords
cationic surfactants
stabilization
enzyme stabilization
stabilize
thermolabile
Prior art date
Application number
BR9911396-1A
Other languages
English (en)
Inventor
John W Shultz
Fen Huang
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of BR9911396A publication Critical patent/BR9911396A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

"ESTABILIZAçãO DE ENZIMA POR MEIO DE TENSOATIVOS CATIÈNICOS". A presente invenção proporciona métodos e composições para a estabilização de proteína, particularmente a estabilização de polimerases em soluções aquosas com tensoativos catiónicos. A presente invenção adicionalmente proporciona tensoativos catiónicos, incluindo aminas polietoxiladas, que estabilizam enzimas termoestáveis e termolábeis em solução. Estes tensoativos estabilizam a atividade das várias enzimas, incluindo as polimerases DNA termoestáveis, polimerases DNA termolábeis e transcriptases reversas.
BR9911396-1A 1998-06-24 1999-06-23 Estabilização de enzima por meio de tensoativos catiÈnicos BR9911396A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9053998P 1998-06-24 1998-06-24
US09/338,174 US6242235B1 (en) 1998-06-24 1999-06-22 Polymerase stabilization by polyethoxylated amine surfactants
PCT/US1999/014217 WO1999067371A1 (en) 1998-06-24 1999-06-23 Enzyme stabilization by cationic surfactants

Publications (1)

Publication Number Publication Date
BR9911396A true BR9911396A (pt) 2002-01-29

Family

ID=26782391

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9911396-1A BR9911396A (pt) 1998-06-24 1999-06-23 Estabilização de enzima por meio de tensoativos catiÈnicos

Country Status (12)

Country Link
US (1) US6242235B1 (pt)
EP (2) EP1918368A3 (pt)
JP (1) JP3673175B2 (pt)
CN (1) CN1188518C (pt)
AT (1) ATE363530T1 (pt)
AU (1) AU761757B2 (pt)
BR (1) BR9911396A (pt)
CA (1) CA2335153C (pt)
DE (1) DE69936201T2 (pt)
ES (1) ES2288317T3 (pt)
HK (1) HK1040262A1 (pt)
WO (1) WO1999067371A1 (pt)

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WO2004042003A2 (en) * 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
ATE550441T1 (de) * 2005-09-06 2012-04-15 Gen Probe Inc Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
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US7846703B2 (en) * 2006-10-02 2010-12-07 Takara Bio Inc. Method for enhancing polymerase activity
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US7972828B2 (en) 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
EP1970440A1 (en) * 2007-03-06 2008-09-17 Qiagen GmbH Polymerase stabilization by ionic detergents
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WO2009006301A2 (en) * 2007-06-29 2009-01-08 Battelle Memorial Institute Protein stabilization
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CN102051352B (zh) * 2009-11-02 2012-07-04 刘萍 一种酶结合物稳定液
CA2806734A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
DK3461807T3 (da) 2011-06-08 2023-09-04 Life Technologies Corp Design og udvikling af hidtil ukendte detergenter til anvendelse i pcr-systemer
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
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RU2636465C2 (ru) 2012-02-09 2017-11-23 Лайф Текнолоджис Корпорейшн Способ конъюгирования полимерной частицы
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WO2014100755A2 (en) * 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
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Also Published As

Publication number Publication date
ES2288317T3 (es) 2008-01-01
DE69936201T2 (de) 2008-02-07
EP1088060A4 (en) 2004-12-01
DE69936201D1 (de) 2007-07-12
CN1315996A (zh) 2001-10-03
EP1088060B1 (en) 2007-05-30
CA2335153C (en) 2007-04-10
JP3673175B2 (ja) 2005-07-20
CN1188518C (zh) 2005-02-09
AU4829399A (en) 2000-01-10
EP1088060A1 (en) 2001-04-04
JP2004500005A (ja) 2004-01-08
AU761757B2 (en) 2003-06-12
ATE363530T1 (de) 2007-06-15
WO1999067371A1 (en) 1999-12-29
EP1918368A2 (en) 2008-05-07
HK1040262A1 (en) 2002-05-31
EP1918368A3 (en) 2008-06-18
CA2335153A1 (en) 1999-12-29
US6242235B1 (en) 2001-06-05

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 13 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.