CN1545042A - Method and apparatus for combining biological microscopic image collection with multimedia teaching - Google Patents

Method and apparatus for combining biological microscopic image collection with multimedia teaching Download PDF

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Publication number
CN1545042A
CN1545042A CNA2003101063033A CN200310106303A CN1545042A CN 1545042 A CN1545042 A CN 1545042A CN A2003101063033 A CNA2003101063033 A CN A2003101063033A CN 200310106303 A CN200310106303 A CN 200310106303A CN 1545042 A CN1545042 A CN 1545042A
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China
Prior art keywords
image
microscope
multimedia teaching
micro
image acquisition
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CNA2003101063033A
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Chinese (zh)
Inventor
杨晓春
丁胜
刘峰
胡茂海
黄琳
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Beijing Qingda Deren Science & Technology Co Ltd
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Beijing Qingda Deren Science & Technology Co Ltd
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Priority to CNA2003101063033A priority Critical patent/CN1545042A/en
Publication of CN1545042A publication Critical patent/CN1545042A/en
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Abstract

The invention is a method of combining bio-microscopic image collection and multimedia instruction. Under the control of a computer, the whole system composed of traditional microscope, relay imaging system, digital shooting system and bio-teaching software completes observing, collecting and processing microscopic images; alternatively uses the software to perform animation simulation, demonstration and interactive response practice. It selects a three-eye microscope, in order to convert optical image into data signals, retains switching with the ocular, and transmits the data signals to the computer by an image conversion interface. It implements real-time monitoring and collection for microscopic images.

Description

The method and apparatus that the biology microscope image acquisition combines with multimedia teaching
One, technical field
The present invention relates to be used for the instrument and the method for bioconversion medium, it is a kind of method and apparatus that utilizes novel digital microsurgical instrument that traditional microtechnic combines with modern digital technology and multimedia bioconversion medium means, especially biology microscope image acquisition to combine with multimedia teaching.
Two, background technology
The method that present bioconversion medium generally adopts has two kinds: 1, directly in the microscopically visualization.2, the image that adopts the simulation camera that microscope is generated is sent to large-screen color TV and observes.The subject matter that adopts these two kinds of methods to exist is: can not dynamically catch micro-image, operations such as real-time storage and graphical analysis.Along with the develop rapidly of computer picture, graph technology and in the widespread use of modern biological field, people need a kind of Real Time Observation, information stores, information analysis and information management integrated novel device system of collecting.
Three, summary of the invention
The objective of the invention is to realize complete operations such as Real Time Observation, motion capture, image storage, projection teaching, graphical analysis to micro-image for bioconversion medium provides an a kind of multi-functional digital micro-analysis instrument and a cover adoption of multimedia teaching means; Dynamically the teaching demonstration main points, editor resources material; Flame Image Process and test result can Projection Display or printouts, also can be used for experiment.The present invention also aims in order fundamentally to overcome the shortcoming of existing method, diagram observation intuitively and digitized processing are combined, the micro-demonstration of image is combined with processing, especially on biomedical sector, use, be also included within the micro image analysis of metal and nonmetallic materials certainly and handle upward application.Detect and analytic system for vast biomedicine, investigation of materials worker provide a kind of novel information, increase work efficiency.And can make integrated software, courseware software and experiment are combined.
The present invention adopts following technical scheme:
The method that the biology microscope image acquisition combines with multimedia teaching, the whole system of forming by conventional microscope, relaying imaging system, digital vedio recording system and bioconversion medium software four parts under the control of computing machine, finish micro-image observation, collection, and handle; And alternately utilize this software to carry out animation simulation, demonstration and the interactivity response practice of education experiment.
Being constructed as follows of bio-edu bioconversion medium software:
1. content of courses selection control
2. teaching method selection control
3. image acquisition and analysis module
4. education experiment animation simulation, demonstration module
5. exercise and discussion animation alternate acknowledge module
Bio-edu bioconversion medium software has following characteristics:
Man-machine interaction friendly interface, main window are used for showing the main contents of study, are furnished with auxiliary window simultaneously and are used for carrying out navigation selection.Can choose at random different learning methods by navigational system.In concrete design, fully adopt multimedia technology, text, chart, sound, animation, video etc. are organically combined, to the student provide a kind of figure, literary composition, sound, as and luxuriant study scene.
Biomedical micro image collection device, biomedical micro image collection instrument is on existing conventional optical microscope basis, be connected with digital camera by interface, especially select trinocular microscope for use, thereby optical image information is converted to data-signal, and the switching of reservation and eyepiece, be transferred in the computing machine by the image transitions interface again.And image information on from the digital camera to the computing machine transmission and the process of processing realize by software programming, method composing images analytic system OptiEye 2.5 of the present invention, according to various actual needs, can carry out micro-image monitoring in real time, image acquisition, Flame Image Process, graphical analysis or the like.
The present invention can intert in courseware software, also can become a sub-portion of courseware software, more can be the processing of independently supporting micro-image.
The method of biomedical micro image collection and processing, the present invention realizes the measurement to the various physical dimensions of micro-image, and the method that image is marked.OptiEye 2.5 makes the user carry out the measurement of various physical dimensions to interested place in the image as required, comprise distance, area etc., by measurement result image is analyzed, can also directly on image, be carried out the mark of arrow and literal simultaneously, make things convenient for the explanation of user image.The flow process See Figure that image is measured.
To select the micro objective enlargement ratio before measuring, and will calibrate earlier.The independent superincumbent right figure of process of calibration provides, and the purpose of calibration is for the actual range on the microscopically image of determining each picture element representative in the image.Just can measure image behind the result after obtaining calibrating, the user selects metering system earlier, and the words that need can also be selected the display mode of measurement result, measure then.
The direct logarithmic code camera of the present invention is operated, and makes the video information in the digital camera can be transferred in the computing machine; Realize one section video flowing is stored or taken to image by software programming, realize monitoring and collection image.
Realize the mark of micro-image by inserting text or direction arrow.
Count the process of the number of particles of pollen by optical microscope, finish the particle statistics of pollen by the method for counting of computer graphics identification automatically.
(1) the real-time monitoring and the collection of realization micro-image.
Having changed traditional microscope use-pattern---necessary health is near microscope, and eyes are pressed close to eyepiece, close-ups, whole people is in bond firmly.And in to micro-image monitoring in real time, can carry out other relevant operations, improved work efficiency greatly, if run into significant image then can store, so that be for further processing.And can take one section video flowing.And the monitoring of image and gather and all to realize by software programming because every kind of camera is all supported the multimedia technology of Microsoft, thus in the program design directly the logarithmic code camera operate, the video information in the digital camera can be transferred in the computing machine.
(2) the various adjustment of realization micro-image, rotation, mirror image, histogram, level and smooth, sharpening.
OptiEye 2.5 provides the interested image that the user is captured to carry out processing such as various adjustment, the shape and the details that can make things convenient for the user better to observe image, thus further understand image.
(3) measurement of realization micro-image physical dimension is to the mark of image.
OptiEye 2.5 makes the user carry out the measurement of various physical dimensions to interested place in the image as required, by measurement result image is analyzed, can also directly on image, carry out simultaneously the mark of arrow and literal, make things convenient for the explanation of user image.
(4) realize that it is automatic identification and statistics with the sterile line cell microscopic image that paddy pollen is educated.
Traditional method is the user counts pollen by ordinary optical microscope a number of particles, this function of OptiEye 2.5 has offered the user finishes pollen automatically by computing machine particle statistics, improved work efficiency greatly, the user has been freed from hard work.
Four, description of drawings
Fig. 1 is a system of the present invention schematic block diagram;
Fig. 2 monitors in real time for the present invention, collection, graphical analysis block diagram;
Fig. 3 is animation simulation of the present invention, demonstration block diagram;
Fig. 4 replys block diagram for interactivity animation of the present invention.
Fig. 5 calibrates process flow diagram for the present invention
Fig. 6 measures process flow diagram for the present invention
Five, embodiment
Biology microscope image collecting device and software systems (referring to Fig. 1):
1. microscope
The microscope of domestic and international multiple model is available.
2. relaying imaging system
Form by relay lens and mechanical interface, be used for the microscopic image that microscope forms is passed to the digital camera imaging surface, microscope and digital camera are fused.
3. digital vedio recording system
Can select the digital camera or the camera of domestic and international multiple model for use.
4.bio-edu bioconversion medium software
1) (for supporting this function, having set up corresponding image data base) is made of at main interface " content of courses selection control ", can enter thematic interface after the selected content of courses; Special topic interface main window is used for showing the main contents of study, is furnished with auxiliary window simultaneously and is used for carrying out navigation selection.
2) navigational system is made up of " teaching method selection control ", is provided with " microscopic examination ", " method and step ", " exercise and discussion " button.At the selected content of courses, can select or change teaching method easily by " teaching method selection control ".
3) activate " microscopic examination " button, the user can obtain the operation interface of " microscopic image is monitored in real time, gathers, handled " module." microscopic image is monitored in real time, collection, processing module " (referring to Fig. 2) development environment is that Microsoft is that the kit DirectX SDK that multimedia systems such as development graph, image, recreation are released mainly comprises: DirectX Graphics, DirectX Audio, DirectInput, DirectPlay, DirectShow, DirectSetup.This module is based on the DirectShow technological development among the DirectX SDK of Microsoft 8.1 editions, the performance brilliance, compatible good, stability is strong.This module comprises following submodule:
A) image acquisition
Activate " image acquisition " module, at first enter the real-time monitor state of micro-image, under this state, the user can capture interested image frame, perhaps catches one section video flowing, and image and video flowing can be preserved, and can also edit and handle image.
B) Flame Image Process
The user can carry out various adjustment to image by software, comprises color, brightness, contrast etc.; Can carry out the processing of sharpening and softening to image; Can also carry out operations such as various convergent-divergents, rotation; Inverse, GTG, embossment, histogram etc. also are the operations that can carry out.
C) graphical analysis
Can measure the physical dimension of image, as length and area; The user can also carry out the mark of literal and arrow to interested place.
4) activate " exercise and discussion " button user and can obtain " content of courses is practised and animation alternate acknowledge module is discussed " operation interface, animation alternate acknowledge module (referring to Fig. 4) chooses a large amount of planes and stereotome embodies visual in image teaching characteristic, the visual effect of rich interface.The ActionScript statement compile script that carries by Flash software is realized the link between the different interactive pages or leaves, and with client's dynamic interaction.Adopt event driven mode to carry out mutual and feedback to user's input reflection.

Claims (7)

1, the method that combines with multimedia teaching of biology microscope image acquisition, the whole system that it is characterized in that forming by conventional microscope, relaying imaging system, digital vedio recording system and bioconversion medium software four parts under the control of computing machine, finish micro-image observation, collection, and handle; And alternately utilize this software to carry out animation simulation, demonstration and the interactivity response practice of education experiment.
2, the method that combines with multimedia teaching by the described biology microscope image acquisition of claim 1, following method is adopted in the measurement that it is characterized in that the logarithmic code shooting: select the micro objective enlargement ratio earlier, and to calibrate earlier, with the actual range on the microscopically image of determining each picture element representative in the image, can carry out various measurements to image after obtaining calibrating the result.
3, the method that is combined with multimedia teaching by the described biology microscope image acquisition of claim 1 is characterized in that the user selects metering system earlier, and selects the display mode of measurement result, measures then.
4, the method that is combined with multimedia teaching by the described biology microscope image acquisition of claim 1 is characterized in that direct logarithmic code camera operates, and makes the video information in the digital camera can be transferred in the computing machine; Realize one section video flowing is stored or taken to image by software programming, realize monitoring and collection image.
5, the method that is combined with multimedia teaching by the described biology microscope image acquisition of claim 1 is characterized in that realizing the mark of micro-image by inserting text or direction arrow.
6, the method that is combined with multimedia teaching by the described biology microscope image acquisition of claim 1 is characterized in that counting by optical microscope the process of the number of particles of pollen, finishes the particle statistics of pollen automatically by the method for counting of computer graphics identification.
7, biomedical micro image collection device, by microscope, relay lens and mechanical interface, digital camera imaging surface, microscope and digital camera are fused, it is characterized in that selecting for use trinocular microscope, thereby optical image information is converted to data-signal, and the switching of reservation and eyepiece, be transferred in the computing machine by the image transitions interface again.
CNA2003101063033A 2003-11-18 2003-11-18 Method and apparatus for combining biological microscopic image collection with multimedia teaching Pending CN1545042A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102622505A (en) * 2012-01-01 2012-08-01 张弢 Fluorescent microscopic image-text collecting and reporting system for autoantibody
CN108205932A (en) * 2018-02-11 2018-06-26 合肥市科技馆 A kind of interactive cell science popularization equipment
CN109029247A (en) * 2018-06-12 2018-12-18 苏州富莱智能科技有限公司 Efficient multi-platform microscopic measuring method
CN110032653A (en) * 2019-03-20 2019-07-19 成都理工大学 One kind is about ground metalliferous mineral visual m odeling technique system
CN112259200A (en) * 2020-11-09 2021-01-22 深圳市爱科学教育科技有限公司 Editing and labeling system and method for microscope experiment result picture

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102622505A (en) * 2012-01-01 2012-08-01 张弢 Fluorescent microscopic image-text collecting and reporting system for autoantibody
CN108205932A (en) * 2018-02-11 2018-06-26 合肥市科技馆 A kind of interactive cell science popularization equipment
CN108205932B (en) * 2018-02-11 2023-10-03 合肥市科技馆 Interactive cell science popularization equipment
CN109029247A (en) * 2018-06-12 2018-12-18 苏州富莱智能科技有限公司 Efficient multi-platform microscopic measuring method
CN110032653A (en) * 2019-03-20 2019-07-19 成都理工大学 One kind is about ground metalliferous mineral visual m odeling technique system
CN112259200A (en) * 2020-11-09 2021-01-22 深圳市爱科学教育科技有限公司 Editing and labeling system and method for microscope experiment result picture

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