FR2362395A1 - METHOD AND COMPOSITION FOR THE DETERMINATION OF GLYCEROL AND TRIGLYCERIDES IN AQUEOUS LIQUIDS - Google Patents

METHOD AND COMPOSITION FOR THE DETERMINATION OF GLYCEROL AND TRIGLYCERIDES IN AQUEOUS LIQUIDS

Info

Publication number
FR2362395A1
FR2362395A1 FR7725353A FR7725353A FR2362395A1 FR 2362395 A1 FR2362395 A1 FR 2362395A1 FR 7725353 A FR7725353 A FR 7725353A FR 7725353 A FR7725353 A FR 7725353A FR 2362395 A1 FR2362395 A1 FR 2362395A1
Authority
FR
France
Prior art keywords
glycerol
triglycerides
determination
composition
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7725353A
Other languages
French (fr)
Other versions
FR2362395B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of FR2362395A1 publication Critical patent/FR2362395A1/en
Application granted granted Critical
Publication of FR2362395B1 publication Critical patent/FR2362395B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé et une composition pour la détection et le dosage du glycérol et des triglycérides dans les liquides aqueux. Le procédé selon l'invention consiste à transformer, dans une premiere étape, le glycérol en L- alpha -glycérophosphate, par exemple au moyen d'une glycérol kinase, puis, dans une deuxieme étape, à oxyder le L- alpha -glycérophosphate au moyen d'une alpha -glycérophosphate oxydase, en presence d'un accepteur d'électrons; on forme ainsi du péroxyde d'hydrogène qu'on utilise pour une réaction quantitative chromogène permettant une mesure colorimétrique. Dans le cas où le liquide aqueux contient des triglycérices, ceux-ci sont d'abord hydrolyses en glycérol, par exemple au moyen d'une lipase. Application au dosage du glycérol et des triglycérides dans le serum sanguin.The invention relates to a method and composition for the detection and assay of glycerol and triglycerides in aqueous liquids. The process according to the invention consists in converting, in a first step, the glycerol into L- alpha -glycerophosphate, for example by means of a glycerol kinase, then, in a second step, in oxidizing the L- alpha -glycerophosphate with by means of an alpha -glycerophosphate oxidase, in the presence of an electron acceptor; hydrogen peroxide is thus formed which is used for a quantitative chromogenic reaction allowing a colorimetric measurement. In the case where the aqueous liquid contains triglycerices, these are first hydrolyzed to glycerol, for example by means of a lipase. Application to the determination of glycerol and triglycerides in the blood serum.

FR7725353A 1976-08-19 1977-08-19 METHOD AND COMPOSITION FOR THE DETERMINATION OF GLYCEROL AND TRIGLYCERIDES IN AQUEOUS LIQUIDS Granted FR2362395A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US71579776A 1976-08-19 1976-08-19

Publications (2)

Publication Number Publication Date
FR2362395A1 true FR2362395A1 (en) 1978-03-17
FR2362395B1 FR2362395B1 (en) 1984-02-24

Family

ID=24875522

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7725353A Granted FR2362395A1 (en) 1976-08-19 1977-08-19 METHOD AND COMPOSITION FOR THE DETERMINATION OF GLYCEROL AND TRIGLYCERIDES IN AQUEOUS LIQUIDS

Country Status (5)

Country Link
JP (3) JPS6012040B2 (en)
CA (1) CA1100023A (en)
DE (1) DE2737288C2 (en)
FR (1) FR2362395A1 (en)
GB (1) GB1590736A (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5545616B2 (en) * 1973-05-26 1980-11-19
US4338395A (en) * 1980-07-21 1982-07-06 Technicon Instruments Corporation Method for the analysis of triglycerides
JPS5783287A (en) * 1980-11-14 1982-05-25 Kyowa Hakko Kogyo Co Ltd Elimination of hydrogen peroxide
JPS5794656A (en) * 1980-12-04 1982-06-12 Konishiroku Photo Ind Co Ltd Element for analysis
JPS5794653A (en) * 1980-12-04 1982-06-12 Konishiroku Photo Ind Co Ltd Element for analysis
JPS60126084A (en) * 1983-12-13 1985-07-05 Toyo Jozo Co Ltd Stabilized glycerohosphate oxidase composition
JP2562882B2 (en) * 1986-12-02 1996-12-11 株式会社シノテスト Stabilization method of reagents for measuring biological components
CN114894668A (en) * 2022-05-11 2022-08-12 山东省分析测试中心 Lipase visual detection method and detection kit based on liquid viscosity change

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2118454A5 (en) * 1970-12-16 1972-07-28 Calbiochem
US3759793A (en) * 1970-01-02 1973-09-18 Boehringer Mannheim Gmbh Process for the quantitative determination of tri di and monoglycerides
FR2266171A1 (en) * 1974-03-25 1975-10-24 Eastman Kodak Co
US3925164A (en) * 1973-03-28 1975-12-09 Boehringer Mannheim Gmbh Method for the determination of cholesterol

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH548029A (en) * 1971-03-30 1974-04-11 Hoffmann La Roche MEANS OF DETECTING GLUCOSE.
JPS4950990A (en) * 1972-09-13 1974-05-17
DK678474A (en) * 1974-02-15 1975-10-13 Hoffmann La Roche

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3759793A (en) * 1970-01-02 1973-09-18 Boehringer Mannheim Gmbh Process for the quantitative determination of tri di and monoglycerides
FR2118454A5 (en) * 1970-12-16 1972-07-28 Calbiochem
US3925164A (en) * 1973-03-28 1975-12-09 Boehringer Mannheim Gmbh Method for the determination of cholesterol
FR2266171A1 (en) * 1974-03-25 1975-10-24 Eastman Kodak Co

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1960 *
CA1969 *

Also Published As

Publication number Publication date
FR2362395B1 (en) 1984-02-24
JPS6012040B2 (en) 1985-03-29
JPH0246200B2 (en) 1990-10-15
JPS5324892A (en) 1978-03-08
JPS5726600A (en) 1982-02-12
DE2737288C2 (en) 1985-05-02
CA1100023A (en) 1981-04-28
DE2737288A1 (en) 1978-02-23
GB1590736A (en) 1981-06-10
JPS61293397A (en) 1986-12-24
JPH0131880B2 (en) 1989-06-28

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