JPH0814B2 - Method and apparatus for culturing liquid phosphorus pieces such as lilies - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and an apparatus for cultivating a piece of phosphorus such as lily using a culture solution.

[0002]

2. Description of the Related Art Lilies are grown on seeds, woods or scaly pieces, but their growth efficiency is extremely low. Particularly, even if the seeds of Yamayuri are collected by self-fertilization, the seeds have an upper cotyledon dormancy phenomenon, and it takes about one and a half years from sowing to germination, so that it takes at least four years before flowering. In recent years, with the selection of excellent varieties of lilies and the development of new varieties, various breeding methods have been developed due to the necessity of mass breeding of the produced lilies. As a method for large-scale growth of lilies, a growth method by tissue culture is mainly used, but in the growth method by tissue culture, a method for dividing bulbous pieces of small bulbs and transplanting them into a new medium to form bulbs and callus is used. There is a method of inducing and proliferating and redifferentiating the bulb.

The method of inducing callus has the advantage that a large amount of callus cells can be proliferated, but the differentiation ability is decreased with the passage of callus. Although it is reliable and there are few mutations, it is difficult to expect high growth efficiency because it grows on an agar medium. Therefore, in order to solve these problems, a method for mass-proliferating phosphorus pieces using a liquid medium was investigated.

As a method for large-scale growth of phosphorus pieces using a liquid medium, liquid shaking culture (Japanese Patent Laid-Open No. 55-11)
No. 8319, Japanese Examined Patent Publication No. 60-15286), a method using a jar-famenter, a method using a horizontal rotary culture tank (Japanese Patent Laid-Open No. 60-232088), and the like. For the purpose of avoiding the damage of the above, a mass-propagation method using a culture tank in which a ventilation tank and a culture tank are independently provided (Japanese Patent Laid-Open No. 62-179383) and a horizontal culture tank provided with a ventilation device on the inner surface of the side wall are used. An improved breeding method such as a large-scale breeding method (Japanese Patent Laid-Open No. 63-192382) has been developed.

On the other hand, an apparatus for culturing by aerating from the bottom of a cylindrical culture container has been used for growing plant cells (Japanese Patent Laid-Open Nos. 2-261373 and 3-160983.
No.), the conventional devices have relatively gentle slopes at the bottom of the culture vessel, and it is unsuitable to apply these devices to the growth of lily pieces.

[0006]

[Problems to be Solved by the Invention] The method for cultivating and growing a piece of scaly flake by liquid culture is a large-scale culture in which oxygen supply by aeration and stirring is good, growth is fast, contact surface with a medium is wide, and nutrient absorption efficiency is good. Although it has various advantages such as being possible, the equipment of the conventional device is difficult,
The culture technique also required various studies. Therefore, an object of the present invention is to provide a method and a device for mass-producing a large amount of scales such as lilies using a scaled liquid culture device that is simple and easy to operate.

[0007]

In order to achieve the above object, the culturing method of the present invention is a method of cultivating a piece of phosphorus, such as a lily, in which the piece of phosphorus is immersed in a culture solution in a culture vessel and cultured with aeration and stirring. In the liquid culture method, the shape of the culture container from the vicinity of the central body to the bottom is made into a substantially inverted truncated cone shape with an acute angle, and aeration is performed from the bottom of the container to culture the phosphorus pieces in a fluidized state. Further, the culture device of the present invention, a culture container, an air supply device,
In a spinach liquid culture device such as a lily equipped with an exhaust device, the shape of the culture container from the vicinity of the central body to the bottom is substantially inverted frustoconical with an acute angle, and the bottom of the container is a bubble-generating porous material. The apparatus is characterized in that the scaly pieces are cultivated in a fluidized state by using an air ejection section formed from a body, a perforated partition wall, and an air diffusion chamber.

[0008] Here, the meaning of "the shape of the culture container from the vicinity of the central body to the bottom is squeezed into an acute angle" means that the perpendicular line perpendicularly extends from the vicinity of the central body of the culture container and the culture container. Is an angle formed with the side wall of the container from the vicinity of the central body portion to the bottom portion (α in FIG. 1), and is preferably 15 to 30 degrees, particularly preferably 17 degrees. Further, at the bottom of the culture vessel, it is desirable that the effective partition wall that defines the air diffusion chamber has at least three or more holes that do not allow phosphorus pieces (about 5 mm) to fall.

[0009]

In the culturing method and the culturing apparatus of the present invention, by making the shape of the culturing vessel to be the above-mentioned specific shape, phosphorus pieces in the culturing solution are floated and fluidized together with the culturing solution without settling at the bottom of the container. In addition, since the smooth flow of the culture solution can be formed, it is possible to culture and grow the phosphorus pieces without the phosphorus pieces colliding with the wall surface of the culture vessel, and thus without damaging the culture tissue.

Further, in the culturing apparatus of the present invention, since the air jetting portion is formed of the bubble-generating porous body, the partition wall having a hole, and the air diffusion chamber, the bubbles generated in the bubble-generating porous body are uniformly distributed in the air diffusion chamber. By diffusing and ejecting this from the holes of the perforated partition wall, air can be ejected over the entire bottom of the culture container, and the phosphorus pieces are prevented from precipitating anywhere on the culture container. The device of the present invention has a very simple structure without the need for a shaking device and a rotating device like the conventional device, and its operation is easy because the ventilation can be controlled. Further, the floating flow of the phosphorus pieces has an effect that the nutrient solution and oxygen can be uniformly supplied in order to efficiently contact the culture solution with the phosphorus pieces.

[0011]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram showing a culture device of the present invention. In FIG. 1, the culture vessel 1 is supported by a support frame 4, and the bottom portion is formed by an air ejection portion 8 having an air ejection hole 9. The culture vessel 1 is made of transparent glass. The air ejection unit 8 communicates with the air pump 10 through the sterilization filter 6 by the air supply pipe 7 to form the air supply device 2. An exhaust pipe 10 extends through the air ejection portion 8 in the culture container 1, and an exhaust port 11 at the tip thereof opens above the liquid surface of the culture solution 13 in the culture container 1.
The exhaust pipe 10 is

Water drop removing device 15 and sterilization filter 14
The exhaust device 3 is formed by opening to the outside of the culture device through the.
In the culture device of the present invention, the inside of the device is kept sterile by the sterilizing filters 6 and 14. In FIG. 2, the air ejection portion 8 of the present invention is formed of a bubble generating porous body 16, an air diffusion chamber 17 and a perforated partition wall 18. Air bubbles generated in the bubble generating porous body 16 are air diffusion chambers. It is uniformly diffused at 17, and is jetted from the air jet holes 9 of the perforated partition wall 18 to the entire bottom of the culture device. The bubble-generating porous body contains
Air stones are used.

The culture container of the present invention is formed such that the shape from the central body portion to the bottom portion is a substantially inverted truncated cone shape with an acute angle. In this embodiment, the shape from the central body portion to the bottom portion of the culture container is The angle (α in FIG. 1) formed between the side wall of the container and the perpendicular line perpendicular to the vicinity of the central body of the culture container is formed to be about 17 degrees. The positional relationship among the air ejection holes 9, the exhaust pipe 10, and the air stones at the bottom of the culture container in the embodiment is as shown in FIG. In this embodiment, the exhaust pipe is provided so as to penetrate through the bottom of the culture container, but the exhaust pipe may be inserted into the container from the upper portion of the culture container and exhausted.

Next, a method of culturing lily pieces, especially the lily pieces of Lily of the valley, using the culturing apparatus having such a structure will be described. Lily pieces can be cultured as they are, but in this Example, solid culture is performed in advance,
The grown one was used. The phosphorus pieces were divided into small pieces, and the surface was sterilized (sodium hypochlorite or ethyl alcohol), and then solid-cultured in Murashige-Skoog medium. In the scaly piece, 1 to 3 progenitors (5 to 1.3 mm, average 0.8 mm) are differentiated in about 3 to 4 months.
The phosphorus pieces were cut into 5 to 10 mm pieces and cultured in the liquid culture device of the present invention. The culture solution was prepared by removing agar from Murashige-Skoog's medium and adding 60 to 9 sucrose.
What was made 0 g / 1 liter was used.

Using a small culture vessel (1 liter), 0.7 liter of the culture solution and about 5 small pieces of porridge of lily of the valley were used.
0 pieces were thrown in. Ventilation drives the air pump 5,
2.5 liters of air were pumped in per minute. The culture was carried out at room temperature (20 to 30 ° C.) for about 60 days. The culture yielded about 1 g of a rapidly-growing lump of the lily, about 1.0-1.6 mm.

[0016]

INDUSTRIAL APPLICABILITY The culturing method and the culturing apparatus of the present invention do not use a shaking or rotating device such as the conventional culturing method and the culturing apparatus, and have a very simple structure, so that a large amount of scale can be grown. Is possible, and the operation is very easy because the culture can be managed simply by controlling the aeration. In addition, in the present invention, by using the culture vessel of a specific structure, a smooth flow of the culture medium is formed, and the phosphorus pieces float and flow without settling at the bottom, and the efficient culture medium The contact can facilitate the rapid growth of the scales. Further, the shape of the culture container has an effect that the phosphorus pieces during the culture do not collide with the wall surface of the container, and therefore the culture tissue is not damaged.

[Brief description of drawings]

FIG. 1 is a schematic view of a culture device of the present invention.

FIG. 2 is a schematic view of an air ejection portion of the culture container shown in FIG.

FIG. 3 shows an example of the positional relationship among the air ejection holes, the exhaust pipe, and the air stones at the bottom of the culture container shown in FIG.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Culture container 5 Air pump 6 Sterilization filter 7 Air supply pipe 8 Air ejection part 9 Air ejection hole 10 Exhaust pipe 14 Sterilization filter 15 Water droplet removal device 16 Air bubble generation porous body 17 Air diffusion chamber 18 Perforated partition wall

Claims (2)

[Claims] To 1. A culture in a culture vessel, crushed immersion phosphorus pieces, in flake liquid culture method such as lily culturing with stirring aeration, the shape of toward the bottom from the vicinity of the center cylinder of the culture vessel, the culture vessel Hung vertically from around the central torso
The vertical line and the volume from near the center of the culture vessel to the bottom
The angle formed with the side wall of the vessel is approximately 15 to 30 degrees.
A method of culturing in a fluidized state, the flakes are cultivated in a fluidized state by forming a substantially inverted truncated cone shape that is squeezed into an acute angle so as to achieve a certain degree, and aerating from the bottom of the container. 2. A culture piece, an air supply device, a lily piece liquid culture apparatus such as a lily provided with an exhaust device, wherein the shape of the culture container from the vicinity of the central body part to the bottom is defined by the center of the culture container.
A vertical line that hangs vertically from around the body and the center body of the culture vessel.
Between the bottom and the side wall of the container
Air jetting part formed by a bubble-generating porous body, a perforated partition wall, and an air diffusion chamber at the bottom of the container, which is formed into a substantially inverted truncated cone shape with an acute angle so that the angle is about 15 to 30 degrees. The device is characterized in that the phosphorus pieces are cultured in a fluidized state.