SE8305327L - INSULATION OF DIFFERENTIATED CELLS - Google Patents

INSULATION OF DIFFERENTIATED CELLS

Info

Publication number
SE8305327L
SE8305327L SE8305327A SE8305327A SE8305327L SE 8305327 L SE8305327 L SE 8305327L SE 8305327 A SE8305327 A SE 8305327A SE 8305327 A SE8305327 A SE 8305327A SE 8305327 L SE8305327 L SE 8305327L
Authority
SE
Sweden
Prior art keywords
dna
sub
cells
sequence
microorganisms
Prior art date
Application number
SE8305327A
Other languages
Swedish (sv)
Other versions
SE8305327D0 (en
Inventor
W J Rutter
H M Goodman
A Ullrich
J Shine
J M Chirgwin
R L Pictet
P H Seeburg
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05897710 external-priority patent/US4363877B1/en
Priority claimed from US05/898,887 external-priority patent/US4264731A/en
Application filed by Univ California filed Critical Univ California
Publication of SE8305327L publication Critical patent/SE8305327L/en
Publication of SE8305327D0 publication Critical patent/SE8305327D0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57518—Placental lactogen; Chorionic somatomammotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Diabetes (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.

Claims (1)

1. Patentkrav Forfarande for isolering av cellar fran Langerhans bar (islet cells), innehallande mRNA, son ar anrikad pa den nukleotidsekvens, som kodar for insulin, kannetecknat av att bukspottkortelvavnad innehallande Oceller och andra cellar behandlas med ett hydrolytiskt enzym och en trypsininhibitor fbr Ott darigenom frigora barna fran.de andra cellerna, den behandlade vavnaden fraktioneras for att darigenom barns shall ernallas i huvudsak trim fran andra celler och fran stinderbnm:tet cellmaterial, Iran dessa bar isolerar man mRNA, vilken ar anrikad pa den nukleotidsekvens, son kodar fbr insulin.A method for isolating cells from Langerhans bar (islet cells), containing mRNA, which is enriched in the nucleotide sequence encoding insulin, characterized in that pancreatic tissue containing Ocells and other cells is treated with a hydrolytic enzyme and a trypsin inhibitor for thereby freeing the children from the other cells, the treated tissue is fractionated so that the children are mainly exposed to trim from other cells and from the stem cell material, Iran these bar isolates mRNA, which is enriched in the nucleotide sequence, which encodes insulin .
SE8305327A 1977-05-27 1983-09-29 INSULATION OF DIFFERENTIATED CELLS SE8305327D0 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US80134377A 1977-05-27 1977-05-27
US80502377A 1977-06-09 1977-06-09
US89770978A 1978-04-19 1978-04-19
US05897710 US4363877B1 (en) 1977-09-23 1978-04-19 Recombinant dna transfer vectors
US05/898,887 US4264731A (en) 1977-05-27 1978-04-21 DNA Joining method
US89900278A 1978-04-26 1978-04-26
US89900378A 1978-04-26 1978-04-26

Publications (2)

Publication Number Publication Date
SE8305327L true SE8305327L (en) 1983-09-29
SE8305327D0 SE8305327D0 (en) 1983-09-29

Family

ID=27569921

Family Applications (4)

Application Number Title Priority Date Filing Date
SE7806086A SE451461B (en) 1977-05-27 1978-05-26 PLASMID WITH THE NUCLEOTIDE SEQUENCE INCLUDES A SEQUENCE CODING FOR A- OR B-CHAIN IN INSULIN AND PROCEDURE FOR ITS PREPARATION AND E.COLI CONTAINING THE PLASMID AND PROCEDURE FOR PRODUCING E.COLI
SE8305327A SE8305327D0 (en) 1977-05-27 1983-09-29 INSULATION OF DIFFERENTIATED CELLS
SE8305329A SE455794B (en) 1977-05-27 1983-09-29 PLASMID WITH NUCLEOTIDE SEQUENCE CODING THE AMINO ACID SEQUENCE IN HUMAN GROWTH HORMON AND PROCEDURE FOR PRODUCING THEREOF
SE8305328A SE8305328D0 (en) 1977-05-27 1983-09-29 ISOLATION OF NON-DRAINED RNA

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SE7806086A SE451461B (en) 1977-05-27 1978-05-26 PLASMID WITH THE NUCLEOTIDE SEQUENCE INCLUDES A SEQUENCE CODING FOR A- OR B-CHAIN IN INSULIN AND PROCEDURE FOR ITS PREPARATION AND E.COLI CONTAINING THE PLASMID AND PROCEDURE FOR PRODUCING E.COLI

Family Applications After (2)

Application Number Title Priority Date Filing Date
SE8305329A SE455794B (en) 1977-05-27 1983-09-29 PLASMID WITH NUCLEOTIDE SEQUENCE CODING THE AMINO ACID SEQUENCE IN HUMAN GROWTH HORMON AND PROCEDURE FOR PRODUCING THEREOF
SE8305328A SE8305328D0 (en) 1977-05-27 1983-09-29 ISOLATION OF NON-DRAINED RNA

Country Status (21)

Country Link
JP (1) JPH0659218B2 (en)
AR (1) AR225404A1 (en)
AU (1) AU521903B2 (en)
BE (1) BE867424A (en)
BG (1) BG40319A3 (en)
CH (1) CH642680A5 (en)
DD (1) DD145927A5 (en)
DE (2) DE2822568A1 (en)
DK (2) DK233278A (en)
FI (1) FI64640C (en)
FR (1) FR2392033B1 (en)
GB (1) GB1565190A (en)
GR (1) GR73552B (en)
IL (1) IL54790A (en)
IT (1) IT1094934B (en)
LU (1) LU79714A1 (en)
NL (1) NL7805591A (en)
NZ (1) NZ187300A (en)
PL (1) PL140200B1 (en)
PT (1) PT68082B (en)
SE (4) SE451461B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA782933B (en) * 1977-09-23 1979-05-30 Univ California Purification of nucleotide sequences suitable for expression in bacteria
RO80052B (en) * 1977-11-08 1985-03-30 Genetech Process for preparing a recombinant cloning vehicle for transforming a bacterial host in order to convert it in heterologous polypeptide generator
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
US6297355B1 (en) 1978-12-22 2001-10-02 Biogen, Inc. Polypeptides displaying HBV antigenicity or hbv antigen specificity
JPS55150883A (en) * 1979-05-11 1980-11-25 Nakano Vinegar Co Ltd Method of storing "mozuku"
GR68404B (en) * 1979-06-01 1981-12-29 Univ California
FR2458584A1 (en) * 1979-06-08 1981-01-02 Pasteur Institut VECTORS FOR THE TRANSFER AND EXPRESSION OF GENETIC MATERIAL IN EUKARYOTE CELL AND METHOD FOR THE PRODUCTION OF A PROTEIN DETERMINED IN EUKARYOTIC CELLS
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
GR70279B (en) * 1979-09-12 1982-09-03 Univ California
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
DE3001928A1 (en) * 1980-01-19 1981-08-06 Hoechst Ag, 6000 Frankfurt GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
CA1202581A (en) * 1980-03-27 1986-04-01 Saran A. Narang Adaptor molecules for dna and their application to synthesis of gene-derived products
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
EP0054331B1 (en) * 1980-12-12 1992-06-03 Unilever N.V. Structural genes encoding the various allelic and maturation forms of preprothaumatin and mutations thereof, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells
IE52417B1 (en) * 1980-12-12 1987-10-28 Unilever Plc Dna sequences encoding various allelic forms of mature thaumatin,recombinant plasmids comprising said dnas and a process for their preparation,bacterial cultures comprising said recombinant plasmids,and method for producing mature thaumatin
US4695543A (en) * 1982-03-23 1987-09-22 Bristol-Myers Company Alpha Interferon GX-1
US4748233A (en) * 1982-03-23 1988-05-31 Bristol-Myers Company Alpha-interferon Gx-1
CA1224432A (en) * 1982-08-17 1987-07-21 Gary N. Buell Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield
US5474980A (en) * 1984-10-04 1995-12-12 Monsanto Company Prolonged release of biologically active somatotropins
US5411951A (en) * 1984-10-04 1995-05-02 Monsanto Company Prolonged release of biologically active somatotropin
DK257988D0 (en) * 1988-05-11 1988-05-11 Novo Industri As NEW PEPTIDES
DE4034702A1 (en) * 1990-10-31 1992-05-07 Siemens Ag METHOD AND DEVICE FOR TESTING THE ACTIVE TOOLS OF DIE BENDING MACHINES, IN PARTICULAR OF BENDING STAMPS

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1521032A (en) * 1974-08-08 1978-08-09 Ici Ltd Biological treatment

Also Published As

Publication number Publication date
FR2392033B1 (en) 1985-09-13
FI64640B (en) 1983-08-31
GR73552B (en) 1984-03-14
IL54790A (en) 1983-07-31
FI64640C (en) 1983-12-12
SE455794B (en) 1988-08-08
JPH0659218B2 (en) 1994-08-10
BG40319A3 (en) 1986-11-14
SE7806086L (en) 1978-11-28
PL207176A1 (en) 1979-03-26
LU79714A1 (en) 1978-11-06
NZ187300A (en) 1982-08-17
SE8305328L (en) 1983-09-29
CH642680A5 (en) 1984-04-30
BE867424A (en) 1978-09-18
DE2858357C2 (en) 1990-03-15
SE8305329L (en) 1983-09-29
FR2392033A1 (en) 1978-12-22
DK791D0 (en) 1991-01-04
IL54790A0 (en) 1978-07-31
AR225404A1 (en) 1982-03-31
JPS5449387A (en) 1979-04-18
SE8305328D0 (en) 1983-09-29
DE2822568C2 (en) 1989-08-31
IT1094934B (en) 1985-08-10
SE451461B (en) 1987-10-12
SE8305327D0 (en) 1983-09-29
PT68082A (en) 1978-06-01
FI781675A (en) 1978-11-28
GB1565190A (en) 1980-04-16
IT7823930A0 (en) 1978-05-29
SE8305329D0 (en) 1983-09-29
AU3648878A (en) 1979-11-29
PL140200B1 (en) 1987-04-30
DE2822568A1 (en) 1978-12-14
NL7805591A (en) 1978-11-29
DK791A (en) 1991-01-04
PT68082B (en) 1980-02-20
DD145927A5 (en) 1981-01-14
DK233278A (en) 1978-11-28
AU521903B2 (en) 1982-05-06

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