SU1707544A1 - Method for detecting occult blood in the biological material - Google Patents

Abstract

The invention relates to medicine, namely to clinical diagnostic studies, and is intended for the rapid and safe determination of blood in feces and other biological material in the diagnosis of internal bleeding. The purpose of the invention is to improve the accuracy of the method for determining, simplifying, isolating the infected material from the environment. The goal is achieved by placing the sample in the wells formed by the plate. Tina with a thickness of 1.5-2 mm, containing holes with a diameter of 10-15 mm, and indicator paper glued to the plate, which is impregnated with a reagent containing 15-20% amidopirin solution, 0.5-27 aniline hydrochloride solution and ethanol 95%, followed by closing the wells with another plate; The resulting kit is placed in a bag of transparent polymeric material and sealed. The manifestation of the reagent is carried out with a solution of hydrogen peroxide introduced by puncture of the bag. The reagent is stabilized by adding to it a preliminarily hydroxylamine solution of 0.1-0.2%. If available in the sample. 0.1-0.2% or more of the blood appears in a bright violet-lilac staining. The method provides high accuracy of determination, reliable isolation of inoculated material from the environment and medical personnel, the use of safe, easily accessible and inexpensive reagents, and ease of use. 1 hp A-ly. (Ј About J ate 4b -u

Description

The invention relates to medicine, namely to clinical diagnostic studies, and is intended for the rapid and safe determination of blood in extrements in the diagnosis of latent intestinal blood formation, and the TRC can also be used to detect latent blood in urine and other biological material. in the examined person with suspected internal bleeding.

- The determination of occult blood is made by a chemical reaction to peroxidase, since hemoglobin has a high pexroxidase activity. The reaction involves a chromogen, an uncolored substance, which in the oxidation process forms a dye, which is an indicator of the reaction. Another essential ingredient is the oxidizing agent. The catalyst is blood homoglobin.

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The purpose of the invention is to increase the accuracy (correctness of the results), simplify the method.

On the basis of a highly effective, safe and recommended reagent for use by the USSR Ministry of Health, inducator paper is created with azoyl powder. For the manufacture of indicator paper, azopiram should be modified compared to its most common option in order to ensure maximum safety of the paper with its high sensitivity.

The composition of azopiram for indicator paper (wt.%): Amidopyrin 15-20

Aniline hydrochloride 0.5-2.0

Ethanol 95 Else

A stabilizer of the following composition is additionally introduced into this reagent, wt%: g-idroxylamine hydrochloride 1.0-2.0; distilled water else.

The stabilizer is introduced into pre-cooked azopiram in an amount of 0.1 of the volume of azopiram. Paper is impregnated with stabilized azopnram. The period of use of indicator paper with azopiram is 12 months, if a stabilizer is introduced into the reagent, and without it - 1 - 2 months.

The basis of the device for detecting hidden blood is perforated plates of white or cream color. For their manufacture, you can use a thick cardboard, plastic, wood (medical spatulas), etc. materials. The optimal dimensions of the plate: length 75-140 mm, width 18-23 mm, thickness 1.5-2.0 mm. Each plate has 1–4 holes 10–15 mm in diameter. The holes are shifted to one end of the plate, and the other, non-perforated end is convenient to take with your hand when using.

These holes are sealed with indicator paper on one side of the plate. Thus, on each plate, wells (recesses) are formed, the edges of which are formed by the edges of the holes in the plates, and the bottom - indicator paper.

Two of these plastics are glued together using paper in pairs of long steps: they form an indicator device that folds like a book cover. On one side (the surface) of this device are the wells. On another surface - indicator bu30

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magician, closing the holes in the plates.

To this folding device is attached a third plate of the same material, but without holes and without paper. This unperformed plate serves as a spatula for applying the material to be tested.

 A kit consisting of a folded indicator device and a spatula are placed in a compartment (pocket) of a bag of thin transparent and colorless polyethylene.

When a sample is taken for analysis, the edge of the adhesive tape sealing one pocket is unscrewed and the indicator device is removed; insert the test material into the recesses of the device (well) so that it touches the indicator paper forming the bottom of the wells. After applying the material to all the wells, the device is folded into the wells, placed in the same pocket of the bag from which the device was removed, and the pocket (excrement) is reinserted between the plates and the clean surface of the test paper and indicator paper is outside. Thus, when the device is pushed back into the pocket, the bag does not get dirty.

The package is used, depending on the number of pockets in it, 1-4 days. Each day material is introduced into the wells of a single indicator device.

To form a reaction to the concealed blood, it is enough to not open the bag, pierce the plastic film and put a few drops of 3% hydrogen peroxide into the pocket, which is the developing solution. If there is blood in the material in the wells, the indicator paper turns violet-violet. If there was no blood, there will be no color reaction.

Example 1. Inspected for intestinal bleeding receives an envelope with the package. There are 3 compartments (pockets) with indicator devices in the package. Each indicator device has 4 wells.

Before the test, the sample of the excreta examined opens one of the plastic bag compartments (pocket) and takes off the adhesive tape. He takes out a set of indicator device, a non-perforated plastin (with a spatula) and an L portion of excrement (from different parts of the total mass of feces) D holes onto the surface of indicator paper. Then, the examined warehouse plate of the device so that the excrement in the wells is located inside the complex and pressed to each other plates. The spatula with which the material was taken is thrown away, and the folded plates with portions of excrement Inside (between them), the patient puts them back into the pocket of the plastic bag and again seals them with the same adhesive tape.15

The next day, the contents of the second pocket are used in a similar way, on the third day - the third pocket.

After the end of the use of a Her-20 tape-lined tape, the package is placed in an envelope on which the necessary information about the subject is written. The envelope with the package is delivered (sent) to the laboratory.

In the laboratory, a plastic bag is removed from the envelope. The laboratory assistant, without opening the bag, shuffles through the polyethylene arm with the end of a glass pi

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A mockup is delivered (sent) to a laboratory, where the indication of blood is carried out as in Example 1.

If necessary, a survey1: ny can perform an analysis on his own, applied 1-2 indicator drops of 3% hydrogen peroxide on a test paper moistened with urine. .The results of the analysis record the HR envelope, which is handed over to the laboratory or doctor.

 The use of the proposed solution provides, in comparison with the known methods, high accuracy (correctness of the results) due to informativity, reliable isolation of infected material from the environment and medical personnel, use of safe in operation, readily available and inexpensive domestic reagents and materials, as well as ease of manufacture and use. .

Claims (2)

1. Method for determining hidden blood in biological material by reacting a sample with a reagent containing amidopirin solution 15-20%, aniline hydrochloride solution 0.5-2% I quote through and inject into each) ethanol 95% followed by a test paper with a few drops of 3% hydrogen peroxide. If the material under study contained 0.1% or more of a sprinkle, immediately or within the next minute, 5 violet-lilac staining appears, clearly visible on the bright Lone of the plates through a transparent film of polyethylene. About the portions of excrements that did not contain blood, staining does not appear .e samples and close the nonperotropic Example 2. Examined for the presence of microhematuria receives an envelope with the package. In the package 3 pockets, in each pocket indicator device. The package is treated in the same way as in Example 1, only in each well not a portion of excrement is introduced, but a drop of urine. Next, an envelope with used plate, the kit is placed in a bag of transparent polymeric material and sealed, and the development is carried out by puncture of the bag. 2. A method according to claim 1, characterized in that, in order to stabilize the reagent, it additionally contains 0.1-0.2% hydroxylamine. Using a hydrogen peroxide solution in the reaction product, characterized in that, in order to isolate the infected material, increase the accuracy and simplify the process, the test paper is impregnated with the reagent, which is glued to a plate with a thickness of 1.5-2.0 mm with holes of 10-15 mm, in the wells formed plate, the kit is placed in a bag of transparent polymeric material and sealed, and the development is carried out by puncture of the bag. 2. A method according to claim 1, characterized in that, in order to stabilize the reagent, it additionally contains 0.1-0.2% hydroxylamine.